Abstract：
BACKGROUND: In reproductive biotechnology, sperm cryopreservation has a vital role to play. Cryopreservation of sperm produces reactive oxygen species (ROS), which disrupt sperm function and structural competence. Numerous protective chemicals, including fructans, have been used during sperm cryopreservation.
OBJECTIVE: To evaluate the effect of different concentrations of the fructosan inulin on ram sperm quality parameters, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) production after freezing and thawing.
METHODS: The pooled samples from four healthy rams were divided into seven equal aliquots and diluted in a Tris-base extender supplemented with 1, 2, 4, 8, 16, and 28 mM of inulin or without inulin supplementation (control). By using liquid nitrogen vapor, the semen was frozen and stored at 196 degree C.
RESULTS: The total motility, viability, and DNA integrity were significantly improved after freeze-thawing with 28 mM inulin, compared to other treatment groups (P < 0.05). A Tris-based extender containing 16 and 28 mM of inulin displayed the highest levels of ram sperm membrane integrity when compared with the control (p <0.05). The abnormality of ram sperm was increased during freeze-thawing at control and 1 mM of inulin, compared to 16 and 28 mM of inulin (P < 0.05). Additionally, 28 mM of inulin decreased MDA and increased SOD activity in ram sperm in comparison with the other treatments (P < 0.05).
CONCLUSIONS: As a result, 28 mM of inulin could be beneficial for the cryopreservation industry and reduce the harmful effects of freeze-thawing on ram sperm. Doi.org/10.54680/fr24510110512.
OBJECTIVE: To evaluate the effect of different concentrations of the fructosan inulin on ram sperm quality parameters, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) production after freezing and thawing.
METHODS: The pooled samples from four healthy rams were divided into seven equal aliquots and diluted in a Tris-base extender supplemented with 1, 2, 4, 8, 16, and 28 mM of inulin or without inulin supplementation (control). By using liquid nitrogen vapor, the semen was frozen and stored at 196 degree C.
RESULTS: The total motility, viability, and DNA integrity were significantly improved after freeze-thawing with 28 mM inulin, compared to other treatment groups (P < 0.05). A Tris-based extender containing 16 and 28 mM of inulin displayed the highest levels of ram sperm membrane integrity when compared with the control (p <0.05). The abnormality of ram sperm was increased during freeze-thawing at control and 1 mM of inulin, compared to 16 and 28 mM of inulin (P < 0.05). Additionally, 28 mM of inulin decreased MDA and increased SOD activity in ram sperm in comparison with the other treatments (P < 0.05).
CONCLUSIONS: As a result, 28 mM of inulin could be beneficial for the cryopreservation industry and reduce the harmful effects of freeze-thawing on ram sperm. Doi.org/10.54680/fr24510110512.
摘要：
背景：在生殖生物技术中，精子冷冻保存具有至关重要的作用。冷冻保存精子会产生活性氧（ROS），破坏精子功能和结构能力。许多保护性化学品，包括果聚糖,已在精子冷冻保存过程中使用。
目的：评价不同浓度菊粉对公羊精子质量参数的影响，超氧化物歧化酶(SOD)活性,冻融后丙二醛（MDA）的产生。
方法:将来自四个健康公羊的合并样品分成七个相等的等分试样,并在补充有1、2、4、8、16和28mM菊粉或不补充菊粉的Tris-碱补充剂中稀释(对照)。通过使用液氮蒸气，精液冷冻保存在196℃。
结果：总运动，生存能力，与28mM菊粉冻融后，DNA完整性显着改善，与其他治疗组比较(P<0.05)。与对照相比,含有16和28mM菊粉的基于Tris的延伸剂显示出最高水平的公羊精子膜完整性(p<0.05)。在对照组和1mM菊粉的冻融过程中，公羊精子的异常增加，与16和28mM菊粉相比（P<0.05）。此外,与其他处理相比，28mM菊粉可降低绵羊精子的MDA和SOD活性（P<0.05）。
结论：因此，28mM的菊粉可能对冷冻保存行业有益，并减少冻融对公羊精子的有害影响。Doi.org/10.54680/fr24510110512。
目的：评价不同浓度菊粉对公羊精子质量参数的影响，超氧化物歧化酶(SOD)活性,冻融后丙二醛（MDA）的产生。
方法:将来自四个健康公羊的合并样品分成七个相等的等分试样,并在补充有1、2、4、8、16和28mM菊粉或不补充菊粉的Tris-碱补充剂中稀释(对照)。通过使用液氮蒸气，精液冷冻保存在196℃。
结果：总运动，生存能力，与28mM菊粉冻融后，DNA完整性显着改善，与其他治疗组比较(P<0.05)。与对照相比,含有16和28mM菊粉的基于Tris的延伸剂显示出最高水平的公羊精子膜完整性(p<0.05)。在对照组和1mM菊粉的冻融过程中，公羊精子的异常增加，与16和28mM菊粉相比（P<0.05）。此外,与其他处理相比，28mM菊粉可降低绵羊精子的MDA和SOD活性（P<0.05）。
结论：因此，28mM的菊粉可能对冷冻保存行业有益，并减少冻融对公羊精子的有害影响。Doi.org/10.54680/fr24510110512。
