PDF(Pubmed)
Abstract：
Chitinase genes, as a class of cell wall hydrolases, are essential for the development and pathogenesis of Fusarium oxysporum f.sp. vasinfectum (F. ox) in cotton, but related research focused on chitinase genes are limited. This study explored two island cotton root secretions from the highly resistant cultivar Xinhai 41 and sensitive cultivar Xinhai 14 to investigate their interaction with F. ox by a weighted correlation network analysis (WGCNA). As a result, two modules that related to the fungal pathogenicity emerged. Additionally, a total of twenty-five chitinase genes were identified. Finally, host-induced gene silencing (HIGS) of FoChi20 was conducted, and the cotton plants showed noticeably milder disease with a significantly lower disease index than the control. This study illuminated that chitinase genes play crucial roles in the pathogenicity of cotton wilt fungi, and the FoChi20 gene could participate in the pathogenesis of F. ox and host-pathogen interactions, which establishes a theoretical framework for disease control in Sea Island cotton.
摘要：
几丁质酶基因，作为一类细胞壁水解酶，对尖孢镰刀菌的发育和发病机理至关重要。血管感染(F.ox)在棉花中，但相关研究集中在几丁质酶基因是有限的。本研究从高抗性品种新海41和敏感品种新海14中探索了两个海岛棉根系分泌物，通过加权相关网络分析（WGCNA）研究了它们与F.ox的相互作用。因此,出现了两个与真菌致病性相关的模块。此外,共鉴定出25个几丁质酶基因。最后,进行FoChi20的宿主诱导基因沉默（HIGS），棉花植株表现出明显温和的病害，病害指数明显低于对照。本研究阐明几丁质酶基因在棉花枯萎病菌的致病性中起着至关重要的作用。FoChi20基因可能参与F.ox的发病机制和宿主-病原体相互作用，建立了海岛棉病害防治的理论框架。
