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Abstract：
This study aimed to investigate the expression of microRNAs (miRNAs) -146b-3p, -221-5p, -222-3p, and -21a-3p and the methylation pattern of the thyroid-stimulating hormone receptor (TSHR) gene in blood plasma samples from papillary thyroid cancer (PTC) patients before and after thyroidectomy compared to healthy controls (HCs). This study included 103 participants, 46 PTC patients and 57 HCs, matched for gender and age. Significantly higher preoperative expression levels of miRNAs and TSHR methylation were determined in the PTC patients compared to HCs. Post-surgery, there was a notable decrease in these biomarkers. Elevated TSHR methylation was linked to larger tumor sizes and lymphovascular invasion, while increased miRNA-222-3p levels correlated with multifocality. Receiver operating characteristic (ROC) analysis showed AUCs below 0.8 for all candidate biomarkers. However, significant changes in the expression of all analyzed miRNAs and TSHR methylation levels indicate their potential to differentiate PTC patients from healthy individuals. These findings suggest that miRNAs and TSHR methylation levels may serve as candidate biomarkers for early diagnosis and monitoring of PTC, with the potential to distinguish PTC patients from healthy individuals. Further research is needed to validate these biomarkers for clinical application.
摘要：
本研究旨在探讨microRNAs(miRNAs)-146b-3p,-221-5p，-222-3p，和-21a-3p以及甲状腺乳头状癌（PTC）患者甲状腺切除术前后血浆样本中促甲状腺激素受体（TSHR）基因的甲基化模式。这项研究包括103名参与者，46名PTC患者和57名HCs，性别和年龄相匹配。与HC相比，PTC患者的miRNA和TSHR甲基化的术前表达水平明显更高。手术后,这些生物标志物显著减少.TSHR甲基化升高与较大的肿瘤大小和淋巴管浸润有关，而miRNA-222-3p水平增加与多灶性相关。接收器操作特征(ROC)分析显示所有候选生物标志物的AUC低于0.8。然而,所有分析的miRNA表达和TSHR甲基化水平的显著变化表明它们有可能区分PTC患者和健康个体。这些结果表明，miRNA和TSHR甲基化水平可作为PTC早期诊断和监测的候选生物标志物。具有区分PTC患者和健康个体的潜力。需要进一步的研究来验证这些生物标志物的临床应用。
