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Abstract：
To explore severity and progression biomarkers, we examined the clinical relevance of multiple cytokines and mediators involved in the inflammatory response in periodontitis. A cohort of 68 patients was enrolled in the study and periodontal status assessed by the current classification of periodontal diseases. Immune mediators present in saliva, of both patients and healthy controls, were quantified using a Legendplex-13 panel. Clinic parameters were significantly higher in PD patients compared with HC, with a strong significant association with the disease severity (stage) (p < 0.001), but not with progression (grade). The panel of immune mediators evidenced elevated levels of pro-inflammatory cytokines IL-6 and IL-1β as disease established (p < 0.01). IL-1β/IL-1RA ratio was increased in PD patients, being associated with disease stage. An anti-inflammatory response was spotted by higher IL-10. Lower levels of IL-23 and IP-10 were associated with disease severity. No significant statistical differences were found by grade classification. Moreover, salivary IL-1β and IL-6 exhibited significant positive correlations with several clinical measurements (PI, BOP, PPD, CAL), while IP-10 showed a statistical negative correlation with BOP, PPD, and CAL. These insights highlight the complexity of the periodontitis inflammatory network and the potential of cytokines as biomarkers for refined diagnostic and therapeutic strategies.
摘要：
为了探索严重程度和进展的生物标志物，我们研究了参与牙周炎炎症反应的多种细胞因子和介质的临床意义。该研究纳入了68名患者的队列，并通过当前的牙周疾病分类评估了牙周状况。唾液中存在的免疫介质，患者和健康对照，使用Legendplex-13面板进行量化。PD患者的临床参数明显高于HC，与疾病严重程度（阶段）有很强的显着关联（p<0.001），但没有进展（等级）。免疫介质组证明随着疾病的确立,促炎细胞因子IL-6和IL-1β的水平升高(p<0.01)。PD患者IL-1β/IL-1RA比值升高,与疾病阶段有关。较高的IL-10发现了抗炎反应。IL-23和IP-10水平较低与疾病严重程度相关。通过等级分类没有发现显著的统计学差异。此外，唾液IL-1β和IL-6与几种临床测量结果呈显著正相关(PI，防喷器,PPD,CAL),而IP-10与BOP呈统计学负相关,PPD,和CAL。这些见解突出了牙周炎炎症网络的复杂性以及细胞因子作为精细诊断和治疗策略的生物标志物的潜力。
