PDF(Pubmed)
Abstract：
The extended-spectrum β-lactamases (ESβLs) are bacterial enzymes capable of hydrolyzing penicillins, cephalosporins, and aztreonam. The prevalence of ESβL is increasing among clinically significant microorganisms worldwide, drastically reducing the therapeutic management of infectious diseases. The study aimed to determine the drug susceptibility of ESβL-positive clinical isolates acquired from patients hospitalized in Lodz, central Poland, and analyze the prevalence of specific genes, determining acquired resistance in these bacteria. The samples of ESβL-positive clinical isolates were gathered in 2022 from medical microbiological laboratories in the city of Lodz, central Poland. The strains were subjected to biochemical identification and antimicrobial susceptibility testing following EUCAST guidelines. The presence of studied genes (blaCTX-M, blaSHV, blaTEM, blaPER, blaVEB) was confirmed by PCR. Over 50% of studied isolates were resistant to gentamicin, cefepime, ceftazidime and ciprofloxacin. The most common ESβL gene was blaCTX-M. In most isolates, the resistance genes occurred simultaneously. The blaPER was not detected in any of the tested strains. ESβL-producing strains are largely susceptible to the currently available antibiotics. The observation of the coexistence of different genes in most clinical isolates is alarming.
摘要：
广谱β-内酰胺酶（ESβLs）是能够水解青霉素的细菌酶，头孢菌素,还有氨曲南.ESβL在全球范围内具有临床意义的微生物中的患病率正在增加，大幅减少传染病的治疗管理。该研究旨在确定从罗兹住院患者中获得的ESβL阳性临床分离株的药物敏感性。波兰中部,并分析特定基因的患病率，确定这些细菌的获得性抗性。2022年从罗兹市的医学微生物实验室收集了ESβL阳性临床分离株的样本，波兰中部。按照EUCAST指南对菌株进行生化鉴定和抗微生物药敏试验。研究基因的存在(blaCTX-M，blaSHV,blaTEM,BlaPER,blaVEB)通过PCR确认。超过50％的研究分离株对庆大霉素具有抗性，头孢吡肟,头孢他啶和环丙沙星.最常见的ESβL基因是blaCTX-M。在大多数分离物中，抗性基因同时发生。在任何测试菌株中均未检测到blaPER。产生ESβL的菌株在很大程度上对目前可用的抗生素敏感。在大多数临床分离株中观察到不同基因的共存是令人震惊的。
