PDF(Pubmed)
Abstract：
Dental plaque bacteria play an important role in the pathogenicity of periodontitis and peri-implantitis. Therefore, antimicrobial agents are one means of treatment. N-chlorotaurine (NCT) as an endogenous well-tolerated topical antiseptic could be of advantage for this purpose. Accordingly, its microbicidal activity against some dental plaque bacteria was investigated at therapeutic concentrations in vitro. In quantitative killing assays, the activity of NCT against planktonic bacteria and against biofilms grown for 48 h on implantation screws was tested. Electron microscopy was used to demonstrate the formation of biofilm and its morphological changes. The killing of planktonic bacteria of all tested species, namely Streptococcus sanguinis, Streptococcus salivarius, Streptococcus oralis, Streptococcus cristatus, Rothia aeria, and Capnocytophaga ochracea, was shown within 10-20 min by 1% NCT in 0.01 M phosphate-buffered saline at 37 °C. Bacteria grown on screws for 24 h were inactivated by 1% NCT after 15-20 min as well, but the formation of biofilm on the screws was visible in electron microscopy not before 48 h. The killing of biofilms by 1% NCT was demonstrated after 30 min (streptococci) and 40 min (R. aeria). As expected, NCT has broad activity against dental plaque bacteria as well and should be further investigated on its clinical efficacy in periodontitis and peri-implantitis.
摘要：
牙菌斑细菌在牙周炎和种植体周炎的致病性中起重要作用。因此,抗菌剂是一种治疗手段。N-氯牛磺酸（NCT）作为内源性耐受性良好的局部防腐剂可能对此具有优势。因此，在体外治疗浓度下研究了其对某些牙菌斑细菌的杀微生物活性。在定量杀伤分析中，测试了NCT对浮游细菌和对植入螺钉上生长48小时的生物膜的活性。电子显微镜用于证明生物膜的形成及其形态变化。杀死所有测试物种的浮游细菌，即血链球菌,唾液链球菌，口链球菌,链球菌的临界状态，Rothiaaeria,和Capnocytophagaochracea，在37°C的0.01M磷酸盐缓冲盐水中通过1%NCT在10-20分钟内显示。15-20分钟后，在螺钉上生长24小时的细菌也被1%NCT灭活，但是在48小时之前，在电子显微镜下可以看到螺钉上生物膜的形成。在30分钟（链球菌）和40分钟（R。aeria）。不出所料,NCT对牙菌斑细菌也具有广泛的活性，应进一步研究其在牙周炎和种植体周炎中的临床疗效。
