PDF(Pubmed)
Abstract：
Parasites have been associated with possible anticancer activity, including Trypanosoma cruzi, which has been linked to inhibiting the growth of solid tumors. To better understand this antitumor effect, we investigated the association of anti-T. cruzi antibodies with B cells of the acute lymphoblastic leukemia (ALL) SUPB15 cell line. The antibodies were generated in rabbits. IgGs were purified by affinity chromatography. Two procedures (flow cytometry (CF) and Western blot(WB)) were employed to recognize anti-T. cruzi antibodies on SUPB15 cells. We also used CF to determine whether the anti-T. cruzi antibodies could suppress SUPB15 cells. The anti-T. cruzi antibodies recognized 35.5% of the surface antigens of SUPB15. The complement-dependent cytotoxicity (CDC) results demonstrate the cross-suppression of anti-T. cruzi antibodies on up to 8.4% of SUPB15 cells. For the WB analysis, a band at 100 kDa with high intensity was sequenced using mass spectrometry, identifying the protein as nucleolin. This protein may play a role in the antitumor effect on T. cruzi. The anti-T. cruzi antibodies represent promising polyclonal antibodies that have the effect of tumor-suppressive cross-linking on cancer cells, which should be further investigated.
摘要：
寄生虫与可能的抗癌活性有关，包括克氏锥虫,这与抑制实体瘤的生长有关。为了更好地理解这种抗肿瘤作用，我们调查了抗T.具有急性淋巴细胞白血病（ALL）SUPB15细胞系B细胞的克鲁兹抗体。在兔中产生抗体。通过亲和层析纯化IgG。采用两种方法（流式细胞术（CF）和蛋白质印迹（WB））来识别抗T。SUPB15细胞上的克鲁兹抗体。我们还使用CF来确定是否抗T。Cruzi抗体可以抑制SUPB15细胞。反T.Cruzi抗体可识别SUPB15表面抗原的35.5％。补体依赖性细胞毒性（CDC）结果表明抗T.Cruzi抗体对高达8.4%的SUPB15细胞。对于WB分析，使用质谱法对100kDa的高强度条带进行测序，鉴定蛋白质为核仁素。该蛋白可能在克氏锥虫的抗肿瘤作用中起作用。反T.Cruzi抗体代表了有希望的多克隆抗体，对癌细胞具有肿瘤抑制交联的作用，应该进一步调查。
