PDF(Pubmed)
Abstract：
Caffeine affords several beneficial effects on human health, acting as an antioxidant, anti-inflammatory agent, and analgesic. Caffeine is widely used in cosmetics, but its antimicrobial activity has been scarcely explored, namely against skin infection agents. Dermatophytes are the most common fungal agents of human infection, mainly of skin infections. This work describes the in vitro effect of caffeine during keratinocyte infection by Trichophyton mentagrophytes, one of the most common dermatophytes. The results show that caffeine was endowed with antidermatophytic activity with a MIC, determined following the EUCAST standards, of 8 mM. Caffeine triggered a modification of the levels of two major components of the fungal cell wall, β-(1,3)-glucan and chitin. Caffeine also disturbed the ultrastructure of the fungal cells, particularly the cell wall surface and mitochondria, and autophagic-like structures were observed. During dermatophyte-human keratinocyte interactions, caffeine prevented the loss of viability of keratinocytes and delayed spore germination. Overall, this indicates that caffeine can act as a therapeutic and prophylactic agent for dermatophytosis.
摘要：
咖啡因对人类健康有多种有益作用，作为抗氧化剂，抗炎剂，和镇痛。咖啡因广泛用于化妆品中，但是它的抗菌活性很少被探索，即针对皮肤感染剂。皮肤癣菌是人类感染最常见的真菌病原体，主要是皮肤感染。这项工作描述了咖啡因在毛癣菌感染角质形成细胞期间的体外作用，最常见的皮肤癣菌之一。结果表明，咖啡因具有MIC的抗皮肤癣菌活性，根据EUCAST标准确定，8mM。咖啡因引发了真菌细胞壁两种主要成分水平的改变，β-(1,3)-葡聚糖和几丁质。咖啡因还扰乱了真菌细胞的超微结构，特别是细胞壁表面和线粒体，并观察到自噬样结构。在皮肤癣菌-人角质形成细胞相互作用期间，咖啡因可防止角质形成细胞的活力丧失和孢子萌发延迟。总的来说,这表明咖啡因可以作为皮肤癣菌病的治疗和预防药物。
