PDF(Pubmed)
Abstract：
Nitrogen is the primary nutrient for plants. Low nitrogen generally affects plant growth and fruit quality. Melon, as an economic crop, is highly dependent on nitrogen. However, the response mechanism of its self-rooted and grafted seedlings to low-nitrogen stress has not been reported previously. Therefore, in this study, we analyzed the transcriptional differences between self-rooted and grafted seedlings under low-nitrogen stress using fluorescence characterization and RNA-Seq analysis. It was shown that low-nitrogen stress significantly inhibited the fluorescence characteristics of melon self-rooted seedlings. Analysis of differentially expressed genes showed that the synthesis of genes related to hormone signaling, such as auxin and brassinolide, was delayed under low-nitrogen stress. Oxidative stress response, involved in carbon and nitrogen metabolism, and secondary metabolite-related differentially expressed genes (DEGs) were significantly down-regulated. It can be seen that low-nitrogen stress causes changes in many hormonal signals in plants, and grafting can alleviate the damage caused by low-nitrogen stress on plants, ameliorate the adverse effects of nitrogen stress on plants, and help them better cope with environmental stresses.
摘要：
氮是植物的主要营养素。低氮通常影响植物生长和果实品质。甜瓜,作为经济作物，高度依赖氮。然而,其自根和嫁接苗对低氮胁迫的响应机制以前没有报道。因此,在这项研究中，我们使用荧光表征和RNA-Seq分析了低氮胁迫下自根和嫁接苗之间的转录差异。结果表明,低氮胁迫显著抑制了甜瓜自根幼苗的荧光特性。差异表达基因的分析表明,与激素信号相关的基因合成,如生长素和油菜素内酯，在低氮胁迫下延迟。氧化应激反应，参与碳和氮代谢，次级代谢产物相关差异表达基因(DEGs)显著下调。可以看出，低氮胁迫引起植物中许多激素信号的变化，嫁接可以减轻低氮胁迫对植物造成的伤害，改善氮胁迫对植物的不利影响，帮助他们更好地应对环境压力。
