PDF(Pubmed)
Abstract：
Colostrum is gaining popularity in cosmetic products. The present study compared the composition and selected biological properties of colostrum from Polish sheep (colostrum 1) and Swiss sheep (colostrum 2), particularly those that can affect healthy or diseased skin. The antioxidant activity of the colostrums was measured using ABTS and DPPH assays. The effect on the proliferation of human skin fibroblasts, neonatal epidermal keratinocytes, and human diabetic fibroblast (dHF) cells isolated from diabetic foot ulcers was also assayed in vitro by MTT and Presto Blue tests, respectively. The colostrum simulated dHF cell proliferation by up to 115.4%. The highest used concentration of colostrum 1 stimulated normal fibroblast proliferation by 191.2% (24 h) and 222.2% (48 h). Both colostrums inhibited epidermal keratinocyte viability. The influence of the colostrums on the expression of genes related to proliferation (Ki67) and immune response (IL-6, PTGS-2, TSG-6) in dHF cells were compared. Colostrum 1 increased the rate of wound closure (scar test). Analysis of total fat, protein and fatty acid content found the Polish colostrum to be a richer source of fat than the Swiss colostrum, which contained a larger amount of protein. Both colostrums exhibit properties that suggest they could be effective components in cosmetic or medicinal formulations for skin care, especially supporting its regeneration, rejuvenation, and wound healing.
摘要：
初乳在化妆品中越来越受欢迎。本研究比较了波兰绵羊（初乳1）和瑞士绵羊（初乳2）的初乳的组成和选定的生物学特性，特别是那些可以影响健康或患病皮肤的。使用ABTS和DPPH测定法测量初乳的抗氧化活性。对人皮肤成纤维细胞增殖的影响，新生儿表皮角质形成细胞，和从糖尿病足溃疡中分离的人糖尿病成纤维细胞（dHF）细胞也通过MTT和PrestoBlue试验在体外进行了测定，分别。初乳模拟dHF细胞增殖高达115.4%。最高使用浓度的初乳1刺激正常成纤维细胞增殖191.2％（24小时）和222.2％（48小时）。两种初乳均抑制表皮角质形成细胞的活力。比较初乳对dHF细胞增殖相关基因(Ki67)和免疫应答相关基因(IL-6、PTGS-2、TSG-6)表达的影响。初乳1增加伤口闭合率(瘢痕试验)。总脂肪分析，蛋白质和脂肪酸含量发现波兰初乳是比瑞士初乳更丰富的脂肪来源，其中含有大量的蛋白质。两种初乳的特性表明它们可能是皮肤护理化妆品或药物制剂中的有效成分，特别是支持它的再生，嫩肤,伤口愈合。
