PDF(Pubmed)
Abstract：
All the nanotechnological devices designed for medical purposes have to deal with the common requirement of facing the complexity of a living organism. Therefore, the development of these nanoconstructs must involve the study of their structural and functional interactions and the effects on cells, tissues, and organs, to ensure both effectiveness and safety. To this aim, imaging techniques proved to be extremely valuable not only to visualize the nanoparticles in the biological environment but also to detect the morphological and molecular modifications they have induced. In particular, histochemistry is a long-established science able to provide molecular information on cell and tissue components in situ, bringing together the potential of biomolecular analysis and imaging. The present review article aims at offering an overview of the various histochemical techniques used to explore the impact of novel nanoproducts as therapeutic, reconstructive and diagnostic tools on biological systems. It is evident that histochemistry has been playing a leading role in nanomedical research, being largely applied to single cells, tissue slices and even living animals.
摘要：
所有用于医疗目的的纳米技术设备都必须满足面对生物体复杂性的共同要求。因此,这些纳米构建体的开发必须涉及它们的结构和功能相互作用以及对细胞的影响的研究，组织，和器官，确保有效性和安全性。为了这个目标,成像技术被证明是非常有价值的，不仅可视化的纳米粒子在生物环境中，而且还检测形态和分子修饰，他们已经诱导。特别是,组织化学是一门历史悠久的科学，能够在原位提供细胞和组织成分的分子信息，结合生物分子分析和成像的潜力。本综述文章旨在概述用于探索新型纳米产品作为治疗药物的影响的各种组织化学技术，生物系统的重建和诊断工具。很明显，组织化学在纳米医学研究中起着主导作用，主要应用于单细胞，组织切片甚至活体动物。
