PDF(Pubmed)
Abstract：
BACKGROUND: In vivo confocal microscopy (IVCM) is a vital tool in studying dry eye disease (DED), providing insights into morphological changes at ocular surface unit levels. This review presents the main differences in corneal structure between aqueous-deficient dry eye disease (AD-DED) and normal eyes.
METHODS: A comprehensive search of PubMed, Web of Science, Embase, and MEDLINE databases from January 2000 to December 2023 was conducted. The study selection process, as well as data selection and examination, were independently performed by two members of the review team.
RESULTS: The review reveals a consistent decrease in corneal surface epithelial cell density in AD-DED cases compared to a control group, but conflicting data on basal epithelial cell density. Notably, the abnormal hyperreflectivity of keratocytes in patients with Sjogren\'s syndrome was recorded, and there was a significant keratocyte density in AD-DED subjects compared to evaporative DED and control groups. Studies also found a decrease in sub-basal nerve density, increased tortuosity, and the fragmentation of nerve fibers. Dendritic cell density and dendritic cell dendrites increase in AD-DED patients compared to healthy subjects.
CONCLUSIONS: IVCM is a powerful tool for enhancing our understanding of the pathophysiological mechanisms underlying DED. However, the review underscores the urgent need to standardize the terminology, analysis, and units used for accurate interpretation, a crucial step in advancing our knowledge of DED.
METHODS: A comprehensive search of PubMed, Web of Science, Embase, and MEDLINE databases from January 2000 to December 2023 was conducted. The study selection process, as well as data selection and examination, were independently performed by two members of the review team.
RESULTS: The review reveals a consistent decrease in corneal surface epithelial cell density in AD-DED cases compared to a control group, but conflicting data on basal epithelial cell density. Notably, the abnormal hyperreflectivity of keratocytes in patients with Sjogren\'s syndrome was recorded, and there was a significant keratocyte density in AD-DED subjects compared to evaporative DED and control groups. Studies also found a decrease in sub-basal nerve density, increased tortuosity, and the fragmentation of nerve fibers. Dendritic cell density and dendritic cell dendrites increase in AD-DED patients compared to healthy subjects.
CONCLUSIONS: IVCM is a powerful tool for enhancing our understanding of the pathophysiological mechanisms underlying DED. However, the review underscores the urgent need to standardize the terminology, analysis, and units used for accurate interpretation, a crucial step in advancing our knowledge of DED.
摘要：
背景：体内共聚焦显微镜（IVCM）是研究干眼病（DED）的重要工具，提供对眼表单位水平形态变化的见解。这篇综述介绍了房水缺乏型干眼症（AD-DED）与正常眼之间角膜结构的主要差异。
方法：对PubMed的全面搜索，WebofScience,Embase,和MEDLINE数据库从2000年1月到2023年12月进行。研究的选择过程，以及数据选择和检查，由审查小组的两名成员独立执行。
结果：该综述显示，与对照组相比，AD-DED病例的角膜表面上皮细胞密度持续下降，但是基底上皮细胞密度的数据相互矛盾。值得注意的是,记录干燥综合征患者角膜细胞的异常高反射率，与蒸发DED和对照组相比，AD-DED受试者的角膜细胞密度显着。研究还发现基底下神经密度下降，弯曲度增加，和神经纤维的碎片。与健康受试者相比，AD-DED患者的树突状细胞密度和树突状细胞树突增加。
结论：IVCM是增强我们对DED的病理生理机制的理解的有力工具。然而,审查强调了术语标准化的迫切需要，分析,和用于准确解释的单位，这是推进我们对DED知识的关键一步。
方法：对PubMed的全面搜索，WebofScience,Embase,和MEDLINE数据库从2000年1月到2023年12月进行。研究的选择过程，以及数据选择和检查，由审查小组的两名成员独立执行。
结果：该综述显示，与对照组相比，AD-DED病例的角膜表面上皮细胞密度持续下降，但是基底上皮细胞密度的数据相互矛盾。值得注意的是,记录干燥综合征患者角膜细胞的异常高反射率，与蒸发DED和对照组相比，AD-DED受试者的角膜细胞密度显着。研究还发现基底下神经密度下降，弯曲度增加，和神经纤维的碎片。与健康受试者相比，AD-DED患者的树突状细胞密度和树突状细胞树突增加。
结论：IVCM是增强我们对DED的病理生理机制的理解的有力工具。然而,审查强调了术语标准化的迫切需要，分析,和用于准确解释的单位，这是推进我们对DED知识的关键一步。
