PDF(Pubmed)
Abstract：
Ischemic events can culminate in acute myocardial infarction, which is generated by irreversible cardiac lesions that cannot be restored due to the limited regenerative capacity of the heart. Cardiac cell therapy aims to replace injured or necrotic cells with healthy and functional cells. Tissue engineering and cardiovascular regenerative medicine propose therapeutic alternatives using biomaterials that mimic the native extracellular environment and improve cellular and tissue functionality. This investigation evaluates the effect of thermosensitive hydrogels, and murine fetal ventricular cardiomyocytes encapsulated in thermosensitive hydrogels, on the contractile function of cardiomyocyte regeneration during an ischemic event. Chitosan and hydrolyzed collagen thermosensitive hydrogels were developed, and they were physically and chemically characterized. Likewise, their biocompatibility was evaluated through cytotoxicity assays by MTT, LDH, and their hemolytic capacity. The hydrogels, and cells inside the hydrogels, were used as an intervention for primary cardiomyocytes under hypoxic conditions to determine the restoration of the contractile capacity by measuring intracellular calcium levels and the expressions of binding proteins, such as a-actinin and connexin 43. These results evidence the potential of natural thermosensitive hydrogels to restore the bioelectrical functionality of ischemic cardiomyocytes.
摘要：
缺血事件可以导致急性心肌梗死，它是由不可逆的心脏损伤产生的，由于心脏的再生能力有限而无法恢复。心脏细胞疗法旨在用健康和有功能的细胞替换受损或坏死的细胞。组织工程和心血管再生医学提出了使用模拟天然细胞外环境并改善细胞和组织功能的生物材料的治疗替代方案。这项研究评估了热敏水凝胶的效果，和小鼠胎儿心室心肌细胞包裹在热敏水凝胶中，缺血事件期间心肌细胞再生的收缩功能。开发了壳聚糖和水解胶原蛋白热敏水凝胶，它们的物理和化学特征。同样，通过MTT细胞毒性试验评估了它们的生物相容性，LDH,和他们的溶血能力。水凝胶，和水凝胶内的细胞，被用作低氧条件下原代心肌细胞的干预，以通过测量细胞内钙水平和结合蛋白的表达来确定收缩能力的恢复，例如a-actinin和连接蛋白43。这些结果证明了天然热敏水凝胶恢复缺血性心肌细胞的生物电功能的潜力。
