PDF(Pubmed)
Abstract：
The toxicity of silver nanoparticles (AgNPs) depends on their physicochemical properties. The ongoing research aims to develop effective methods for modifying AgNPs using molecules that enable control over the processes induced by nanoparticles in both normal and cancerous cells. Application of amino acid-stabilized nanoparticles appears promising, exhibiting tunable electrokinetic properties. Therefore, this study focused on determining the influence of the surface charge of cysteine (CYS)-stabilized AgNPs on their toxicity towards human normal B (COLO-720L) and T (HUT-78) lymphocyte cell lines. CYS-AgNPs were synthesized via the chemical reduction. Transmission electron microcopy (TEM) imaging revealed that they exhibited a quasi-spherical shape with an average size of 18 ± 3 nm. CYS-AgNPs remained stable under mild acidic (pH 4.0) and alkaline (7.4 and 9.0) conditions, with an isoelectric point observed at pH 5.1. Following a 24 h treatment of lymphocytes with CYS-AgNPs, concentration-dependent alterations in cell morphology were observed. Positively charged CYS-AgNPs notably decreased lymphocyte viability. Furthermore, they exhibited grater genotoxicity and more pronounced disruption of biological membranes compared to negatively charged CYZ-AgNPs. Despite both types of AgNPs interacting similarly with fetal bovine serum (FBS) and showing comparable profiles of silver ion release, the biological assays consistently revealed that the positively charged CYS-AgNPs exerted stronger effects at all investigated cellular levels. Although both types of CYS-AgNPs have the same chemical structure in their stabilizing layers, the pH-induced alterations in their surface charge significantly affect their biological activity.
摘要：
银纳米粒子(AgNPs)的毒性取决于它们的物理化学性质。正在进行的研究旨在开发使用分子修饰AgNPs的有效方法，这些分子能够控制正常细胞和癌细胞中纳米粒子诱导的过程。氨基酸稳定的纳米颗粒的应用似乎很有前景，表现出可调的电动特性。因此,这项研究的重点是确定半胱氨酸（CYS）稳定的AgNPs的表面电荷对其对人类正常B（COLO-720L）和T（HUT-78）淋巴细胞系的毒性的影响。通过化学还原合成CYS-AgNP。透射电子显微镜（TEM）成像显示，它们表现出平均尺寸为18±3nm的准球形。CYS-AgNPs在弱酸性（pH4.0）和碱性（7.4和9.0）条件下保持稳定，在pH5.1时观察到等电点。用CYS-AgNPs处理淋巴细胞24小时后，观察到细胞形态的浓度依赖性改变。带正电荷的CYS-AgNP显著降低淋巴细胞活力。此外，与带负电荷的CYZ-AgNP相比，它们表现出更大的遗传毒性和更明显的生物膜破坏。尽管两种类型的AgNPs与胎牛血清（FBS）相互作用相似，并且显示出银离子释放的可比曲线，生物测定一致显示,带正电荷的CYS-AgNPs在所有研究的细胞水平上都发挥了更强的作用.尽管两种类型的CYS-AgNP在其稳定层中具有相同的化学结构，pH诱导的表面电荷变化显着影响其生物活性。
