关键词: Phyllosticta citricarpa antifungal activity biomarkers metabolomic profiling susceptibility

Mesh : Biomarkers Plant Diseases / microbiology Citrus / chemistry microbiology Ascomycota / chemistry Oils, Volatile / pharmacology chemistry Gas Chromatography-Mass Spectrometry Antifungal Agents / pharmacology chemistry Acyclic Monoterpenes / pharmacology chemistry Metabolome

来  源:   DOI:10.3390/molecules29153582   PDF(Pubmed)

Abstract:
Citrus black spot (CBS) is a fungal disease caused by Phyllosticta citricarpa Kiely, (McAlpine Van der Aa), with most cultivars being susceptible to infection. Currently, disease control is based on the application of protective fungicides, which is restricted due to resistance, health and environmental concerns. Although using natural products for disease management is gaining momentum, more advances are required. This study obtained the metabolic profiles of the essential oil and cuticular waxes of two citrus cultivars with a varying susceptibility to CBS infection using gas chromatography-mass spectrometry. A multivariate data analysis identified possible biomarker compounds that contributed to the difference in susceptibility between the two cultivars. Several identified biomarkers were tested in vitro for their antifungal properties against P. citricarpa. Two biomarkers, propanoic acid and linalool, were able to completely inhibit pathogen growth at 750 mg/L and 2000 mg/L, respectively.
摘要:
柑橘黑斑(CBS)是由柑橘毛竹引起的真菌病,(McAlpineVanderAa),大多数品种容易受到感染。目前,疾病控制是基于保护性杀菌剂的应用,由于阻力而受到限制,健康和环境问题。尽管使用天然产品进行疾病管理的势头正在增强,需要更多的进步。这项研究使用气相色谱-质谱法获得了两种柑橘品种的精油和角质层蜡的代谢谱,这些柑橘品种对CBS感染的敏感性不同。多变量数据分析确定了可能的生物标志物化合物,这些化合物导致了两个品种之间的易感性差异。在体外测试了几种鉴定的生物标记物的抗真菌性质。两种生物标志物,丙酸和芳樟醇,能在750mg/L和2000mg/L下完全抑制病原体生长,分别。
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