PDF(Pubmed)
Abstract：
Fabiana punensis S. C. Arroyo is a subshrub or shrub that is indigenous to the arid and semiarid region of northern Argentina and is known to possess several medicinal properties. The objective of this study was to optimize the extraction conditions so as to maximize the yield of bioactive total phenolic compound (TPC) and flavonoids (F) of F. punensis\' aerial parts by using non-conventional extraction methods, namely ultrasound-assisted extraction, UAE, and microwave-assisted extraction, MAE, and to compare the biological activities and toxicity of optimized extracts vs. conventional extracts, i.e., those gained by maceration. Response Surface Methodology (RSM) was used to apply factorial designs to optimize the parameters of extraction: solid-to-liquid ratio, extraction time, ultrasound amplitude, and microwave power. The experimental values for TPC and F and antioxidant activity under the optimal extraction conditions were not significantly different from the predicted values, demonstrating the accuracy of the mathematical models. Similar HPLC-DAD patterns were found between conventional and UAE- and MAE-optimized extracts. The main constituents of the extracts correspond to phenolic compounds (flavonoids and phenolic acids) and apigenin was identified. All extracts showed high scavenger capacity on ABTS•+, O2•- and H2O2, enabling the inhibition of the pro-inflammatory enzymes xanthine oxidase (XO) and lipoxygenase (LOX). They also showed an antimutagenic effect in Salmonella Typhimurium assay and cytotoxic/anti-proliferative activity on human melanoma cells (SKMEL-28). Toxicological evaluation indicates its safety. The results of this work are important in the development of efficient and sustainable methods for obtaining bioactive compounds from F. punensis for the prevention of chronic degenerative diseases associated with oxidative stress, inflammation, and DNA damage.
摘要：
FabianapunensisS.C.Arroyo是一种亚灌木或灌木，原产于阿根廷北部的干旱和半干旱地区，已知具有多种药用特性。本研究的目的是优化提取条件，以最大限度地获得生物活性总酚类化合物（TPC）和黄酮类化合物（F）通过使用非常规提取方法。即超声辅助提取，阿联酋，和微波辅助提取，MAE,并比较优化提取物的生物活性和毒性常规提取物,即，那些通过浸渍获得的。响应面法（RSM）用于应用析因设计来优化提取参数：固液比，提取时间,超声振幅,微波功率。最优提取条件下的TPC和F及抗氧化活性的实验值与预测值无显著差异,证明了数学模型的准确性。在常规和UAE和MAE优化的提取物之间发现了相似的HPLC-DAD模式。提取物的主要成分对应于酚类化合物（类黄酮和酚酸），并鉴定了芹菜素。所有提取物在ABTS•+上显示出高清除剂能力，O2•-和H2O2，能够抑制促炎酶黄嘌呤氧化酶（XO）和脂氧合酶（LOX）。它们还在鼠伤寒沙门氏菌测定中显示出抗突变作用和对人黑素瘤细胞的细胞毒性/抗增殖活性(SKMEL-28)。毒理学评价表明其安全性。这项工作的结果对于开发有效和可持续的方法非常重要，可持续的方法可以从Punensis获得生物活性化合物，以预防与氧化应激相关的慢性退行性疾病，炎症,和DNA损伤。
