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Abstract：
This article presents a new method for preparing multifunctional composite biomaterials with applications in advanced biomedical fields. The biomaterials consist of dicalcium phosphate (DCPD) and bioactive silicate glasses (SiO2/Na2O and SiO2/K2O), containing the antibiotic streptomycin sulfate. Materials were deeply characterized by X-ray diffraction and attenuated total reflectance Fourier transform infrared spectroscopy, and zeta potential analysis, UV-visible spectrophotometry, and ion-exchange measurement were applied in a simulating body fluid (SBF) solution. The main results include an in situ chemical transformation of dicalcium phosphate into an apatitic phase under the influence of silicate solutions and the incorporation of the antibiotic. The zeta potential showed a decrease in surface charge from ζ = -24.6 mV to ζ = -16.5 mV. In addition, a controlled and prolonged release of antibiotics was observed over a period of 37 days, with a released concentration of up to 755 ppm. Toxicity tests in mice demonstrated good tolerance of the biomaterials, with no significant adverse effects. Moreover, these biomaterials have shown potent antibacterial activity against various bacterial strains, including Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, suggesting their potential use in tissue engineering, drug delivery, and orthopedic and dental implants. By integrating the antibiotic into the biomaterial composites, we achieved controlled release and prolonged antibacterial efficacy. This research contributes to advancing biomaterials by exploring innovative synthetic routes and showcasing their promise in regenerative medicine and controlled drug delivery.
摘要：
本文提出了一种制备多功能复合生物材料的新方法，并将其应用于先进的生物医学领域。生物材料由磷酸二钙（DCPD）和生物活性硅酸盐玻璃（SiO2/Na2O和SiO2/K2O）组成，含有抗生素硫酸链霉素。通过X射线衍射和衰减全反射傅里叶变换红外光谱对材料进行了深入表征,和zeta电位分析，紫外可见分光光度法,在模拟体液（SBF）溶液中进行离子交换测量。主要结果包括在硅酸盐溶液的影响下将磷酸二钙原位化学转化为磷灰石相和抗生素的掺入。ζ电位显示表面电荷从ζ=-24.6mV降低至ζ=-16.5mV。此外,在37天的时间内观察到抗生素的受控和延长释放，释放浓度高达755ppm。小鼠毒性试验证明了对生物材料的良好耐受性，无明显不良反应。此外，这些生物材料对各种细菌菌株显示出有效的抗菌活性，包括单核细胞增生李斯特菌,金黄色葡萄球菌,大肠杆菌,铜绿假单胞菌,表明它们在组织工程中的潜在用途，药物输送,以及骨科和牙科植入物。通过将抗生素整合到生物材料复合材料中，我们实现了控制释放和延长抗菌疗效。这项研究通过探索创新的合成路线并展示其在再生医学和受控药物递送方面的前景，为推进生物材料的发展做出了贡献。
