PDF(Pubmed)
Abstract：
Medicinal plant-derived vesicle-like nanoparticles can carry chemical components and exert intercellular activity due to the encapsulation of nanostructures. American ginseng is well known as a traditional herb and is commonly used in clinical decoctions. However, the nano-characteristics and chemical composition of American-ginseng-derived vesicle-like nanoparticles (AGVNs) in decoctions are unclear. In this study, the gradient centrifugation method was used to extract and isolate AGVNs. A metabolomic method based on high-resolution mass spectrometry was established to analyze small molecules loaded in AGVNs. Zebrafish and RAW264.7 cells were employed to investigate the anti-inflammatory effects of AGVNs. The results showed that the particle size of AGVNs was generally 243.6 nm, and the zeta potential was -14.5 mV. AGVNs were found to contain 26 ginsenosides (14 protopanaxadiols, 11 protopanaxatriols, and 1 oleanolic acid). Ginsenoside Rb1 and malonyl-ginsenoside Rb1 tended to be enriched in AGVNs. Moreover, AGVNs were found to exert anti-inflammatory effects by reducing macrophage migration in zebrafish and regulating inflammatory factor (NO, TNF-α, IL-6, IL-10) secretion in RAW 264.7 cells. The characterization and analysis of AGVNs provide references and data that support the development of nanoscale anti-inflammatory substances from medicinal plants.
摘要：
药用植物来源的囊泡状纳米颗粒可以携带化学成分，并由于纳米结构的包封而发挥细胞间活性。西洋参是众所周知的传统草药，常用于临床汤剂中。然而,西洋参来源的囊泡状纳米颗粒（AGVN）在汤剂中的纳米特征和化学成分尚不清楚。在这项研究中,采用梯度离心法提取和分离AGVN。建立了一种基于高分辨率质谱的代谢组学方法来分析负载在AGVN中的小分子。使用斑马鱼和RAW264.7细胞来研究AGVN的抗炎作用。结果表明,AGVNs的粒径一般为243.6nm,zeta电位为-14.5mV。发现AGVN含有26种人参皂苷（14种原人参二醇，11原参三醇，和1个齐墩果酸)。人参皂苷Rb1和丙二酰人参皂苷Rb1趋于富集在AGVN中。此外，发现AGVNs通过减少斑马鱼巨噬细胞迁移和调节炎症因子而发挥抗炎作用(NO,TNF-α,IL-6，IL-10）在RAW264.7细胞中的分泌。AGVNs的表征和分析提供了支持从药用植物中开发纳米级抗炎物质的参考和数据。
