PDF(Pubmed)
Abstract：
Waxy maize is mainly cultivated in South Korea for the production of food and snacks, and colored maize with increased anthocyanin content is used in the production of functional foods and medicinal products. Association mapping analysis (AMA) is supported as the preferred method for identifying genetic markers associated with complex traits. Our study aimed to identify molecular markers associated with two anthocyanin content and six seed coat color traits in near-isogenic lines (NILs) of colored waxy maize assessed through AMA. We performed AMA for 285 SSR loci and two anthocyanin content and six seed coat color traits in 10 NILs of colored waxy maize. In the analysis of population structure and cluster formation, the two parental lines (HW3, HW9) of \"Mibaek 2ho\" variety waxy maize and the 10 NILs were clearly divided into two groups, with each group containing one of the two parental inbred lines. In the AMA, 62 SSR markers were associated with two seed anthocyanin content and six seed coat color traits in the 10 NILs. All the anthocyanin content and seed coat color traits were associated with SSR markers, ranging from 2 to 12 SSR markers per characteristic. The 12 SSR markers were together associated with both of the two anthocyanin content (kuromanin and peonidin) traits. Our current results demonstrate the effectiveness of SSR analysis for the examination of genetic diversity, relationships, and population structure and AMA in 10 NILs of colored waxy maize and the two parental lines of the \"Mibaek 2ho\" variety waxy maize.
摘要：
糯玉米主要在韩国种植，用于生产食品和小吃，花青素含量增加的有色玉米用于生产功能性食品和药用产品。支持关联作图分析(AMA)作为鉴定与复杂性状相关的遗传标记的优选方法。我们的研究旨在鉴定通过AMA评估的有色糯玉米近等基因系（NIL）中与两种花色苷含量和六种种皮颜色性状相关的分子标记。我们对10个有色糯玉米NIL中的285个SSR位点和两个花色苷含量和六个种皮颜色性状进行了AMA。在人口结构和集群形成的分析中，“Mibaek2ho”品种糯玉米的两个亲本系（HW3，HW9）和10个NIL明确分为两组，每组包含两个亲本近交系之一。在AMA中,在10个NILs中，62个SSR标记与2个种子花色苷含量和6个种皮颜色性状相关。所有花色苷含量和种皮颜色性状均与SSR标记相关，每个特征范围从2到12个SSR标记。12个SSR标记与两个花色苷含量（kuromanin和牡丹）性状都相关。我们目前的结果证明了SSR分析对遗传多样性检查的有效性，关系,10个有色糯玉米和“Mibaek2ho”品种糯玉米的两个亲本系的种群结构和AMA。
