PDF(Pubmed)
Abstract：
Callus induction (CI) is a critical trait for transforming desirable genes in plants. A genome-wide association study (GWAS) analysis was conducted on the rice germplasms of 110 Indica rice accessions, in which three tissue culture media, B5, MS, and N6, were used for the CI of those rice panels\' mature seeds. Seven quantitative trait loci (QTLs) on rice chromosomes 2, 6, 7, and 11 affected the CI percentage in the three media. For the B5 medium, one QTL (qCI-B5-Chr6) was identified on rice chromosome 6; for the MS medium, two QTLs were identified on rice chromosomes 2 and 6 (qCI-MS-Chr2 and qCI-MS-Chr6, respectively); for the N6 medium, four QTLs were identified on rice chromosomes 6, 7, and 11 (qCI-N6-Chr6.1 and qCI-N6-Chr6.2, qCI-N6-Chr7, and qCI-N6-Chr11, respectively). Fifty-five genes were identified within the haplotype blocks corresponding to these QTLs, thirty-one of which showed haplotypes associated with different CI percentages in those media. qCI-B5-Chr6 was located in the same region as qCI-N6-Chr6.2, and the Caleosin-related family protein was also identified in this region. Analysis of the gene-based haplotype revealed the association of this gene with different CI percentages in both B5 and N6 media, suggesting that the gene may play a critical role in the CI mechanism. Moreover, several genes, including those that encode the beta-tubulin protein, zinc finger protein, RNP-1 domain-containing protein, and lysophosphatidic acid acyltransferase, were associated with different CI percentages in the N6 medium. The results of this study provide insights into the potential QTLs and candidate genes for callus induction in rice that contribute to our understanding of the physiological and biochemical processes involved in callus formation, which is an essential tool in the molecular breeding of rice.
摘要：
愈伤组织诱导(CI)是植物中转化所需基因的关键性状。对110份粳稻种质资源进行了全基因组关联研究(GWAS)分析,其中三种组织培养基，B5,MS,和N6，用于这些水稻组成熟种子的CI。水稻2、6、7和11号染色体上的七个数量性状基因座（QTLs）影响了三种培养基中的CI百分比。对于B5培养基，在水稻6号染色体上鉴定出一个QTL（qCI-B5-Chr6）；对于MS培养基，在水稻2号和6号染色体上鉴定出两个QTL（分别为qCI-MS-Chr2和qCI-MS-Chr6）；对于N6培养基，在水稻染色体6、7和11上鉴定出四个QTL（分别为qCI-N6-Chr6.1和qCI-N6-Chr6.2，qCI-N6-Chr7和qCI-N6-Chr11）。在与这些QTL相对应的单倍型区块内鉴定出55个基因，其中31个显示与这些培养基中不同CI百分比相关的单倍型。qCI-B5-Chr6位于与qCI-N6-Chr6.2相同的区域中,并且在该区域中还鉴定出与Caleosin相关的家族蛋白。基于基因的单倍型分析揭示了该基因与B5和N6培养基中不同CI百分比的关联。提示该基因可能在CI机制中起关键作用。此外，几个基因，包括那些编码β-微管蛋白的蛋白，锌指蛋白,含RNP-1结构域的蛋白质，和溶血磷脂酸酰基转移酶，与N6培养基中不同的CI百分比相关。这项研究的结果提供了对水稻愈伤组织诱导的潜在QTL和候选基因的见解，这有助于我们对愈伤组织形成所涉及的生理和生化过程的理解，是水稻分子育种的重要工具。
