PDF(Pubmed)
Abstract：
This study presents a portable, low-cost, point-of-care (POC) system for the simultaneous detection of blood glucose and hematocrit. The system consists of a disposable origami microfluidic paper-based analytical device (μPAD) for plasma separation, filtration, and reaction functions and a 3D-printed cassette for hematocrit and blood glucose detection using a smartphone. The origami μPAD is patterned using a cost-effective label printing technique instead of the conventional wax printing method. The 3D-printed cassette incorporates an array of LED lights, which mitigates the effects of intensity variations in the ambient light and hence improves the accuracy of the blood glucose and hematocrit concentration measurements. The hematocrit concentration is determined quantitatively by measuring the distance of plasma wicking along the upper layer of the origami μPAD, which is pretreated with sodium chloride and Tween 20 to induce dehydration and aggregation of the red blood cells. The filtered plasma also penetrates to the lower layer of the origami μPAD, where it reacts with embedded colorimetric assay reagents to produce a yellowish-brown complex. A color image of the reaction complex is captured using a smartphone inserted into the 3D-printed cassette. The image is analyzed using self-written RGB software to quantify the blood glucose concentration. The calibration results indicate that the proposed detection platform provides an accurate assessment of the blood glucose level over the range of 45-630 mg/dL (R2 = 0.9958). The practical feasibility of the proposed platform is demonstrated by measuring the blood glucose and hematocrit concentrations in 13 human whole blood samples. Taking the measurements obtained from commercial glucose and hematocrit meters as a benchmark, the proposed system has a differential of no more than 6.4% for blood glucose detection and 9.1% for hematocrit detection. Overall, the results confirm that the proposed μPAD is a promising solution for cost-effective and reliable POC health monitoring.
摘要：
这项研究提出了一种便携式的，低成本,即时（POC）系统，用于同时检测血糖和血细胞比容。该系统由用于血浆分离的一次性折纸微流体纸基分析设备（μPAD）组成，过滤,和反应功能以及使用智能手机进行血细胞比容和血糖检测的3D打印盒。使用具有成本效益的标签印刷技术而不是传统的蜡印刷方法对折纸μPAD进行图案化。3D打印的盒子包含一系列LED灯，这减轻了环境光中的强度变化的影响,并且因此提高了血糖和血细胞比容浓度测量的准确性。通过测量沿着折纸μPAD上层的血浆芯吸距离来定量确定血细胞比容浓度，用氯化钠和吐温20预处理以诱导红细胞的脱水和聚集。过滤后的等离子体也渗透到折纸μPAD的下层，在那里它与嵌入的比色测定试剂反应以产生黄棕色复合物。使用插入3D打印盒中的智能手机捕获反应复合物的彩色图像。使用自写的RGB软件分析图像以量化血糖浓度。校准结果表明,所提出的检测平台提供了对45-630mg/dL范围内的血糖水平的准确评估(R2=0.9958)。通过测量13种人全血样品中的血糖和血细胞比容浓度来证明所提出的平台的实际可行性。以从商用葡萄糖和血细胞比容仪获得的测量结果作为基准，该系统的血糖检测差异不超过6.4%，血细胞比容检测差异不超过9.1%。总的来说,结果证实，所提出的μPAD是具有成本效益和可靠的POC健康监测的有前途的解决方案。
