PDF(Pubmed)
Abstract：
Recently, the therapeutic potential of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) has been extensively studied in both human and veterinary medicine. EVs are nano-sized particles containing biological components commonly found in other biological materials. For that reason, EV isolation and characterization are critical to draw precise conclusions during their investigation. Research on EVs within veterinary medicine is still considered in its early phases, yet numerous papers were published in recent years. The conventional adult tissues for deriving MSCs include adipose tissue and bone marrow. Nonetheless, alternative sources such as synovial fluid, endometrium, gingiva, and milk have also been intermittently used. Fetal adnexa are amniotic membrane/fluid, umbilical cord and Wharton\'s jelly. Cells derived from fetal adnexa exhibit an intermediate state between embryonic and adult cells, demonstrating higher proliferative and differentiative potential and longer telomeres compared to cells from adult tissues. Summarized here are the principal and recent preclinical and clinical studies performed in domestic animals such as horse, cattle, dog and cat. To minimize the use of antibiotics and address the serious issue of antibiotic resistance as a public health concern, they will undoubtedly also be utilized in the future to treat infections in domestic animals. A number of concerns, including large-scale production with standardization of EV separation and characterization techniques, must be resolved for clinical application.
摘要：
最近,来自间充质干细胞（MSC）的细胞外囊泡（EV）的治疗潜力已在人类和兽医学中得到广泛研究。EV是包含通常在其他生物材料中发现的生物组分的纳米尺寸的颗粒。出于这个原因,电动汽车的隔离和表征对于在调查期间得出准确的结论至关重要。兽医领域的电动汽车研究仍处于早期阶段，然而，近年来发表了许多论文。用于衍生MSC的常规成人组织包括脂肪组织和骨髓。尽管如此，替代来源，如滑液，子宫内膜,牙龈,和牛奶也被间歇性地使用。胎儿附件是羊膜/液体，脐带和沃顿的果冻。来自胎儿附件的细胞表现出胚胎和成体细胞之间的中间状态，与成年组织细胞相比，具有更高的增殖和分化潜能和更长的端粒。这里总结了主要的和最近的临床前和临床研究在家畜如马，牛,狗和猫尽量减少抗生素的使用，并解决作为公共卫生问题的抗生素耐药性这一严重问题，毫无疑问，它们将来也将用于治疗家畜的感染。一些担忧,包括电动汽车分离和表征技术标准化的大规模生产，必须解决临床应用。
