METHODS: Six groups of Kunming mice (control, Baijiu injury, silybin, low, medium, and high) were modelled by gavage with Baijiu (for 14 days) and PPC (equivalent to a maximum dose of 9 g/kg in humans). The liver transcriptome data were analyzed to predict gene annotation, followed by the verification of gut microbiota, serum, tissue staining, immunohistochemistry, and Western blotting. Liquid chromatography-mass spectrometry was used to detect the components.
RESULTS: PPC normalized serum ALT (40 U/L), down-regulated TLR4-NF-κB signaling pathway to inhibit the release of TNF-α (90 pg/mL), improved the expression of occludin, claudin-4, and ZO-1, and restored the abundance of Muribaculaceae, Bacteroides and Streptococcus.
CONCLUSIONS: PPC can alleviate ALD by regulating the gut microbiota with an anti-inflammatory and intestinal barrier, and has an application value in developing functional foods.
方法:6组昆明种小鼠(对照,白酒受伤,水飞蓟宾,低,中等,和高)通过用白酒(14天)和PPC(相当于人的最大剂量为9g/kg)进行建模。分析肝脏转录组数据以预测基因注释,其次是肠道微生物群的验证,血清,组织染色,免疫组织化学,和西方印迹。采用液相色谱-质谱联用技术检测各组分。
结果:PPC标准化血清ALT(40U/L),下调TLR4-NF-κB信号通路抑制TNF-α(90pg/mL)释放,改善了occludin的表达,claudin-4和ZO-1,并恢复了杂草科的丰富,拟杆菌和链球菌。
结论:PPC可以通过调节肠道菌群,抗炎和肠屏障来缓解ALD,在开发功能性食品方面具有应用价值。