PDF(Pubmed)
Abstract：
BACKGROUND: Lipid metabolism disorder appears to be one of the early features of alcoholic liver disease (ALD), which can be speculated via omics analysis including liver transcriptomics and gut microbiota. A complex consisting of the roots of Pueraria lobata and dried fruits of Prunus mume (PPC), which possesses hepatoprotective effects, could serve as a drug or functional food. The lack of non-polysaccharide compounds in PPC with their moderation effects on gut microbiota suggests the necessity for a relevant study.
METHODS: Six groups of Kunming mice (control, Baijiu injury, silybin, low, medium, and high) were modelled by gavage with Baijiu (for 14 days) and PPC (equivalent to a maximum dose of 9 g/kg in humans). The liver transcriptome data were analyzed to predict gene annotation, followed by the verification of gut microbiota, serum, tissue staining, immunohistochemistry, and Western blotting. Liquid chromatography-mass spectrometry was used to detect the components.
RESULTS: PPC normalized serum ALT (40 U/L), down-regulated TLR4-NF-κB signaling pathway to inhibit the release of TNF-α (90 pg/mL), improved the expression of occludin, claudin-4, and ZO-1, and restored the abundance of Muribaculaceae, Bacteroides and Streptococcus.
CONCLUSIONS: PPC can alleviate ALD by regulating the gut microbiota with an anti-inflammatory and intestinal barrier, and has an application value in developing functional foods.
METHODS: Six groups of Kunming mice (control, Baijiu injury, silybin, low, medium, and high) were modelled by gavage with Baijiu (for 14 days) and PPC (equivalent to a maximum dose of 9 g/kg in humans). The liver transcriptome data were analyzed to predict gene annotation, followed by the verification of gut microbiota, serum, tissue staining, immunohistochemistry, and Western blotting. Liquid chromatography-mass spectrometry was used to detect the components.
RESULTS: PPC normalized serum ALT (40 U/L), down-regulated TLR4-NF-κB signaling pathway to inhibit the release of TNF-α (90 pg/mL), improved the expression of occludin, claudin-4, and ZO-1, and restored the abundance of Muribaculaceae, Bacteroides and Streptococcus.
CONCLUSIONS: PPC can alleviate ALD by regulating the gut microbiota with an anti-inflammatory and intestinal barrier, and has an application value in developing functional foods.
摘要：
背景：脂质代谢紊乱似乎是酒精性肝病（ALD）的早期特征之一，这可以通过组学分析来推测，包括肝脏转录组学和肠道微生物群。由葛根和李子干果实（PPC）组成的复合体，具有保肝作用，可以作为药物或功能性食品。PPC中缺乏非多糖化合物及其对肠道微生物群的调节作用表明有必要进行相关研究。
方法：6组昆明种小鼠(对照,白酒受伤,水飞蓟宾,低,中等,和高）通过用白酒（14天）和PPC（相当于人的最大剂量为9g/kg）进行建模。分析肝脏转录组数据以预测基因注释，其次是肠道微生物群的验证，血清,组织染色，免疫组织化学，和西方印迹。采用液相色谱-质谱联用技术检测各组分。
结果：PPC标准化血清ALT（40U/L），下调TLR4-NF-κB信号通路抑制TNF-α(90pg/mL)释放,改善了occludin的表达，claudin-4和ZO-1，并恢复了杂草科的丰富，拟杆菌和链球菌。
结论：PPC可以通过调节肠道菌群，抗炎和肠屏障来缓解ALD，在开发功能性食品方面具有应用价值。
方法：6组昆明种小鼠(对照,白酒受伤,水飞蓟宾,低,中等,和高）通过用白酒（14天）和PPC（相当于人的最大剂量为9g/kg）进行建模。分析肝脏转录组数据以预测基因注释，其次是肠道微生物群的验证，血清,组织染色，免疫组织化学，和西方印迹。采用液相色谱-质谱联用技术检测各组分。
结果：PPC标准化血清ALT（40U/L），下调TLR4-NF-κB信号通路抑制TNF-α(90pg/mL)释放,改善了occludin的表达，claudin-4和ZO-1，并恢复了杂草科的丰富，拟杆菌和链球菌。
结论：PPC可以通过调节肠道菌群，抗炎和肠屏障来缓解ALD，在开发功能性食品方面具有应用价值。
