PDF(Pubmed)
Abstract：
Fermented foods and ingredients, including furmenties derived from lactic acid bacteria (LAB) in dairy products, can modulate the immune system. Here, we describe the use of reconstituted skimmed milk powder to generate novel fermentates from Lactobacillus helveticus strains SC232, SC234, SC212, and SC210, and from Lacticaseibacillus casei strains SC209 and SC229, and demonstrate, using in vitro assays, that these fermentates can differentially modulate cytokine secretion via bone-marrow-derived dendritic cells (BMDCs) when activated with either the viral ligand loxoribine or an inflammatory stimulus, lipopolysaccharide. Specifically, we demonstrate that SC232 and SC234 increase cytokines IL-6, TNF-α, IL-12p40, IL-23, IL-27, and IL-10 and decrease IL-1β in primary bone-marrow-derived dendritic cells (BMDCs) stimulated with a viral ligand. In contrast, exposure of these cells to SC212 and SC210 resulted in increased IL-10, IL-1β, IL-23, and decreased IL-12p40 following activation of the cells with the inflammatory stimulus LPS. Interestingly, SC209 and SC229 had little or no effect on cytokine secretion by BMDCs. Overall, our data demonstrate that these novel fermentates have specific effects and can differentially enhance key immune mechanisms that are critical to viral immune responses, or can suppress responses involved in chronic inflammatory conditions, such as ulcerative colitis (UC), and Crohn\'s disease (CD).
摘要：
发酵食品和配料，包括乳制品中来自乳酸菌（LAB）的毛皮，可以调节免疫系统。这里,我们描述了使用重组脱脂奶粉从瑞士乳杆菌菌株SC232，SC234，SC212和SC210以及干酪乳杆菌菌株SC209和SC229产生新型发酵物，并证明了，使用体外测定法，这些发酵物可以通过骨髓衍生的树突状细胞（BMDCs），当被病毒配体洛沙滨或炎症刺激物激活时，脂多糖。具体来说,我们证明SC232和SC234增加细胞因子IL-6，TNF-α，IL-12p40，IL-23，IL-27和IL-10减少用病毒配体刺激的原代骨髓衍生的树突状细胞（BMDC）中的IL-1β。相比之下,这些细胞暴露于SC212和SC210导致增加的IL-10，IL-1β，IL-23和在用炎症刺激物LPS激活细胞后降低IL-12p40。有趣的是,SC209和SC229对BMDC的细胞因子分泌几乎没有或没有影响。总的来说,我们的数据表明，这些新的发酵物具有特定的作用，可以不同地增强关键的免疫机制，是关键的病毒免疫反应，或者可以抑制慢性炎症反应，如溃疡性结肠炎（UC），和克罗恩病（CD）。
