PDF(Pubmed)
Abstract：
This study delves into the potential of amorphous titanium oxide (aTiO2) nano-coating to enhance various critical aspects of non-Ti-based metallic orthopedic implants. These implants, such as medical-grade stainless steel (SS), are widely used for orthopedic devices that demand high strength and durability. The aTiO2nano-coating, deposited via magnetron sputtering, is a unique attempt to improve the osteogenesis, the inflammatory response, and to reduce bacterial colonization on SS substrates. The study characterized the nanocoated surfaces (SS-a TiO2) in topography, roughness, wettability, and chemical composition. Comparative samples included uncoated SS and sandblasted/acid-etched Ti substrates (Ti). The biological effects were assessed using human mesenchymal stem cells (MSCs) and primary murine macrophages. Bacterial tests were carried out with two aerobic pathogens (S. aureusandS. epidermidis) and an anaerobic bacterial consortium representing an oral dental biofilm. Results from this study provide strong evidence of the positive effects of the aTiO2nano-coating on SS surfaces. The coating enhanced MSC osteoblastic differentiation and exhibited a response similar to that observed on Ti surfaces. Macrophages cultured on aTiO2nano-coating and Ti surfaces showed comparable anti-inflammatory phenotypes. Most significantly, a reduction in bacterial colonization across tested species was observed compared to uncoated SS substrates, further supporting the potential of aTiO2nano-coating in biomedical applications. The findings underscore the potential of magnetron-sputtering deposition of aTiO2nano-coating on non-Ti metallic surfaces such as medical-grade SS as a viable strategy to enhance osteoinductive factors and decrease pathogenic bacterial adhesion. This could significantly improve the performance of metallic-based biomedical devices beyond titanium.
摘要：
这项研究探讨了无定形氧化钛（aTiO2）纳米涂层的潜力，以增强非Ti基金属骨科植入物的各种关键方面。这些植入物,如医疗级不锈钢(SS)，广泛用于需要高强度和耐久性的矫形装置。TiO2纳米涂层，通过磁控溅射沉积，是改善成骨的独特尝试，炎症反应，并减少SS底物上的细菌定植。＆＃xD;该研究在形貌中表征了纳米涂层表面（SS-aTiO2），粗糙度,润湿性,和化学成分。对比样品包括未涂覆的SS和喷砂/酸蚀刻的Ti基底(Ti)。使用人间充质干细胞（MSC）和原代鼠巨噬细胞评估生物学效应。对两种需氧病原体进行了细菌测试(S.金黄色葡萄球菌和表皮葡萄球菌）和代表口腔牙齿生物膜的厌氧细菌聚生体。＆＃xD;这项研究的结果为TiO2纳米涂层对SS表面的积极作用提供了有力的证据。涂层增强了MSC成骨细胞的分化，并表现出与Ti表面相似的反应。在TiO2纳米涂层和Ti表面上培养的巨噬细胞显示出相当的抗炎表型。最重要的是，与未涂覆的SS底物相比，观察到在测试物种中细菌定植的减少，进一步支持TiO2纳米涂层在生物医学应用中的潜力。＆＃xD;这些发现强调了在非Ti金属表面（如医用级SS）上磁控溅射沉积TiO2纳米涂层的潜力，作为增强骨诱导因子和减少病原菌粘附的可行策略。这可以显着提高金属基生物医学设备的性能，而不是钛。
