Abstract:
Cellular senescence is a cell fate triggered in response to stress and is characterized by stable cell-cycle arrest and a hypersecretory state. It has diverse biological roles, ranging from tissue repair to chronic disease. The development of new tools to study senescence in vivo has paved the way for uncovering its physiological and pathological roles and testing senescent cells as a therapeutic target. However, the lack of specific and broadly applicable markers makes it difficult to identify and characterize senescent cells in tissues and living organisms. To address this, we provide practical guidelines called \"minimum information for cellular senescence experimentation in vivo\" (MICSE). It presents an overview of senescence markers in rodent tissues, transgenic models, non-mammalian systems, human tissues, and tumors and their use in the identification and specification of senescent cells. These guidelines provide a uniform, state-of-the-art, and accessible toolset to improve our understanding of cellular senescence in vivo.
摘要:
细胞衰老是响应应激而触发的细胞命运,其特征在于稳定的细胞周期停滞和过度分泌状态。它具有不同的生物学作用,从组织修复到慢性疾病。体内研究衰老的新工具的开发为揭示其生理和病理作用以及测试衰老细胞作为治疗靶标铺平了道路。然而,缺乏特异性和广泛适用的标记使得难以鉴定和表征组织和活生物体中的衰老细胞。为了解决这个问题,我们提供了称为“体内细胞衰老实验的最低信息”(MICSE)的实用指南。它介绍了啮齿动物组织中衰老标记的概述,转基因模型,非哺乳动物系统,人体组织,和肿瘤及其在鉴定和鉴定衰老细胞中的用途。这些指南提供了制服,最先进的,和可访问的工具集,以提高我们对体内细胞衰老的理解。