Abstract：
A novel self-powered and flexible enzymatic biofuel cell (EBFC)-based aptasensor was developed for the sensitive and selective detection of 17 β-estradiol (E2). A flexible polyvinyl alcohol (PVA)-tannic acid‑carbon nanotube/reduced graphene oxide (PTCR) substrate was modified with gold nanoparticles (AuNPs) and thiolated aptamer 1 (Apt1) to yield Apt1@AuNPs/PTCR. A copper-based metal-organic framework (Cu-MOF) with peroxidase mimicking activity was employed to anchor glucose oxidase (GOD) and Apt2, forming the Cu-MOF@GOD/Apt2 tag. When E2 was recognized by Apt1, the anchored E2 quantitatively recognized Cu-MOF@GOD/Apt2 to create a Cu-MOF@GOD/Apt2-E2-Apt1 sandwich structure for glucose oxidation to generate electrical power. Increased E2 concentrations enhanced Cu-MOF@GOD/Apt2 capture and amplified the electrical signal. The electrical power increased linearly as the E2 concentration increased from 1.0 pM to 1.0 nM. The sensor was successfully applied to various food samples and blood serum detection. This work promoted the application of novel self-powered biosensors for food safety analysis.
摘要：
开发了一种新型的自供电且灵活的基于酶促生物燃料电池（EBFC）的aptasensor，用于灵敏和选择性地检测17β-雌二醇（E2）。用金纳米粒子（AuNP）和巯基化适体1（Apt1）修饰柔性聚乙烯醇（PVA）-单宁酸-碳纳米管/还原氧化石墨烯（PTCR）基底，以产生Apt1@AuNP/PTCR。具有过氧化物酶模拟活性的铜基金属有机骨架(Cu-MOF)用于锚定葡萄糖氧化酶(GOD)和Apt2,形成Cu-MOF@GOD/Apt2标签。当E2被Apt1识别时，锚定的E2定量识别Cu-MOF@GOD/Apt2以创建Cu-MOF@GOD/Apt2-E2-Apt1夹心结构，用于葡萄糖氧化以产生电力。增加的E2浓度增强了Cu-MOF@GOD/Apt2捕获并放大了电信号。随着E2浓度从1.0μM增加到1.0nM,电功率线性增加。该传感器已成功应用于各种食品样品和血清检测。这项工作促进了新型自供电生物传感器在食品安全分析中的应用。
