Abstract：
Aquaculture farming generates a significant amount of wastewater, which has prompted the development of creative bioprocesses to improve wastewater treatment and bioresource recovery. One promising method of achieving these aims is to directly recycle pollutants into microbe-rice bran complexes, which is an economical and efficient technique for wastewater treatment that uses synergetic interactions between algae and bacteria. This study explores novel bioaugmentation as a promising strategy for efficiently forming microbial-rice bran complexes in unsterilized aquaculture wastewater enriched with agricultural residues (molasses and rice bran). Results found that rice bran serves a dual role, acting as both an alternative nutrient source and a biomass support for microalgae and bacteria. Co-bioaugmentation, involving the addition of probiotic bacteria (Bacillus syntrophic consortia) and microalgae consortiums (Tetradesmus dimorphus and Chlorella sp.) to an existing microbial community, led to a remarkable 5-fold increase in microbial-rice bran complex yields compared to the non-bioaugmentation approach. This method provided the most compact biofloc structure (0.50 g/L) and a large particle diameter (404 μm). Co-bioaugmentation significantly boosts the synthesis of extracellular polymeric substances, comprising proteins at 6.5 g/L and polysaccharides at 0.28 g/L. Chlorophyta, comprising 80% of the total algal phylum, and Proteobacteria, comprising 51% of the total bacterial phylum, are emerging as dominant species. These microorganisms play a crucial role in waste and wastewater treatment, as well as in the formation of microbial-rice bran complexes that could serve as an alternative aquaculture feed. This approach prompted changes in both microbial community structure and nutrient cycling processes, as well as water quality. These findings provide valuable insights into the transformative effects of bioaugmentation on the development of microbial-rice bran complexes, offering potential applications in bioprocesses for waste and wastewater management.
摘要：
水产养殖会产生大量的废水，这促进了创新生物工艺的发展，以改善废水处理和生物资源回收。实现这些目标的一种有希望的方法是将污染物直接再循环到微生物-米糠复合物中，这是一种经济有效的废水处理技术，它利用藻类和细菌之间的协同作用。这项研究探索了新颖的生物强化作为一种有前途的策略，可在富含农业残留物（糖蜜和米糠）的未灭菌水产养殖废水中有效形成微生物-米糠复合物。结果发现米糠具有双重作用，作为微藻和细菌的替代营养来源和生物质支持。共同生物强化，涉及添加益生菌（互养芽孢杆菌）和微藻（Tetradesmusdimorphus和小球藻。)到现有的微生物群落，与非生物强化方法相比，微生物-米糠复合物的产量显着提高了5倍。该方法提供了最致密的biofloc结构（0.50g/L）和大粒径（404μm）。协同生物强化显著促进了细胞外聚合物的合成，包含6.5g/L的蛋白质和0.28g/L的多糖。绿藻门,占藻类门总数的80%，和变形杆菌，占细菌门总数的51%，正在成为优势物种。这些微生物在废物和废水处理中起着至关重要的作用，以及形成可用作替代水产养殖饲料的微生物米糠复合物。这种方法促进了微生物群落结构和养分循环过程的变化，以及水质。这些发现为生物强化对微生物米糠复合物发育的转化作用提供了有价值的见解，在废物和废水管理的生物过程中提供潜在的应用。
