Abstract：
Molecular Recognition in nucleotides is crucial for medicine, underpinning precise interactions in genetic replication and therapy. Alkylated nucleotides, in particular, play a key role in modifying DNA to inhibit cancer cell growth. In this study, we focused on an alkylated nucleotide, PNM2 (3\',4\',6\'-O-tristearoyl uridine or uridine tri-stearate), to investigate the interaction between adenine molecules in the aqueous subphase and PNM2 Langmuir monolayers. Utilizing techniques such as tensiometry, Brewster angle microscopy, infrared spectroscopy, surface potential measurements, and dilatational surface rheology, we found compelling evidence of molecular Recognition between the polar head of the insoluble amphiphile (uridine) in the monolayer and adenine in the aqueous subphase, attributed to hydrogen bonding. These interactions significantly influenced the physicochemical properties of the air-water interface, including monolayer expansion upon molecular recognition, decreased dilatational modulus, increased tensiometric stability of the monolayer when compressed to relevant surface pressures, and decreased surface potential. These findings are noteworthy for drug development, providing crucial insights into the mechanisms of nucleotide interactions.
摘要：
核苷酸的分子识别对医学至关重要，支持基因复制和治疗中的精确相互作用。烷基化核苷酸，特别是,在修饰DNA以抑制癌细胞生长中起关键作用。在这项研究中,我们专注于烷基化核苷酸，PNM2(3',4\',6'-O-三硬脂酰尿苷或尿苷三硬脂酸酯)，研究水亚相中腺嘌呤分子与PNM2Langmuir单层之间的相互作用。利用张力计等技术，布鲁斯特角显微镜,红外光谱,表面电位测量，和膨胀表面流变学，我们发现了在单层中不溶性两亲物（尿苷）的极性头和水亚相中的腺嘌呤之间的分子识别的令人信服的证据，归因于氢键。这些相互作用显着影响了空气-水界面的物理化学性质，包括分子识别后的单层扩展，膨胀模量降低，当压缩到相关的表面压力时，单层的拉伸稳定性增加，表面电位降低。这些发现对于药物开发来说是值得注意的，提供对核苷酸相互作用机制的重要见解。
