Abstract：
Prostate cancer (PCa) is a common male malignancy and early diagnosis is crucial for successful treatment. The current study aims to validate results from a pilot study that demonstrated an inverse association between urine tyrosine and tryptophan levels and the severity of PCa. This study comprised a cohort of 97 patients with benign prostatic hyperplasia, 93 patients diagnosed with localized PCa, 75 patients diagnosed with locally advanced PCa, and 68 patients diagnosed with metastatic PCa. The tyrosine and tryptophan levels in the samples were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and electrochemical sensors in accordance with the pilot to maintain uniformity for accurately evaluating the data. One-way ANOVA with post Tukey test as well as the Wilcoxon Rank Sum Test were performed. Analyzing 333 patients across PCa stages with consistent methods, we observed no significant differences in tyrosine and tryptophan levels between PCa patients and controls, finally rejecting the use of tyrosine and tryptophan as PCa biomarkers. We did, however, verify the strong correlation between the urinary concentrations of tyrosine and tryptophan found in the pilot study.
摘要：
前列腺癌（PCa）是一种常见的男性恶性肿瘤，早期诊断对成功治疗至关重要。本研究旨在验证一项初步研究的结果，该研究证明了尿酪氨酸和色氨酸水平与PCa严重程度之间的负相关。这项研究包括97名良性前列腺增生患者，93例诊断为局限性PCa，诊断为局部晚期PCa的75例患者，68例患者诊断为转移性PCa。样品中的酪氨酸和色氨酸水平通过液相色谱-串联质谱(LC-MS/MS)和电化学传感器根据试验进行分析,以保持均匀性以准确评估数据。进行了具有Tukey后测试以及Wilcoxon等级和测试的单向ANOVA。用一致的方法分析333例PCa分期患者，我们观察到PCa患者和对照组之间的酪氨酸和色氨酸水平没有显着差异，最终拒绝使用酪氨酸和色氨酸作为PCa生物标志物。我们做到了,然而,验证在初步研究中发现的酪氨酸和色氨酸的尿浓度之间的强相关性。
