Abstract：
The vaginal microbiome (VM) is associated with human papillomavirus (HPV) infection and progression, but a thorough understanding of the relation between HPV infection, and VM needs to be elucidated. From August to December 2022, women who underwent routine gynecological examinations were screened for HPV infection. The distribution of HPV variants and clinical characteristics were collected. Then, a total of 185 participants were enrolled and divided into HPV-negative (HC), high-risk HPV (H), low-risk HPV (L), multiple high-risk HPV (HH), and mixed high-low risk HPV (HL) groups. Samples were collected from the mid-vagina of these 185 participants and sent for 16S rDNA sequencing (V3-V4 region). Among 712 HPV-positive women, the top 3 most frequently detected genotypes were HPV52, HPV58, and HPV16. Among 185 participants in the microbiology study, the β diversity of the HC group was significantly different from HPV-positive groups (P < 0.001). LEfSe analysis showed that Lactobacillus iners was a potential biomarker for H group, while Lactobacillus crispatus was for L group. Regarding HPV-positive patients, the α diversity of cervical lesion patients was remarkably lower than those with normal cervix (P < 0.05). Differential abundance analysis showed that Lactobacillus jensenii significantly reduced in cervical lesion patients (P < 0.001). Further community state type (CST) clustering displayed that CST IV was more common than other types in HC group (P < 0.05), while CST I was higher than CST IV in H group (P < 0.05). Different HPV infections had distinct vaginal microbiome features. HPV infection might lead to the imbalance of Lactobacillus spp. and cause cervical lesions.
OBJECTIVE: In this study, we first investigated the prevalence of different HPV genotypes in south China, which could provide more information for HPV vaccinations. Then, a total of 185 subjects were selected from HPV-negative, high-risk, low-risk, multiple hr-hr HPV infection, and mixed hr-lr HPV infection populations to explore the vaginal microbiome changes. This study displayed that HPV52, HPV58, and HPV16 were the most prevalent high-risk variants in south China. In addition, high-risk HPV infection was featured by Lactobacillus iners, while low-risk HPV infection was by Lactobacillus crispatus. Further sub-group analysis showed that Lactobacillus jensenii was significantly reduced in patients with cervical lesions. Finally, CST clustering showed that CST IV was the most common type in HC group, while CST I accounted the most in H group. In a word, this study for the first time systemically profiled vaginal microbiome of different HPV infections, which may add bricks to current knowledge on HPV infection and lay the foundation for novel treatment/prevention development.
OBJECTIVE: In this study, we first investigated the prevalence of different HPV genotypes in south China, which could provide more information for HPV vaccinations. Then, a total of 185 subjects were selected from HPV-negative, high-risk, low-risk, multiple hr-hr HPV infection, and mixed hr-lr HPV infection populations to explore the vaginal microbiome changes. This study displayed that HPV52, HPV58, and HPV16 were the most prevalent high-risk variants in south China. In addition, high-risk HPV infection was featured by Lactobacillus iners, while low-risk HPV infection was by Lactobacillus crispatus. Further sub-group analysis showed that Lactobacillus jensenii was significantly reduced in patients with cervical lesions. Finally, CST clustering showed that CST IV was the most common type in HC group, while CST I accounted the most in H group. In a word, this study for the first time systemically profiled vaginal microbiome of different HPV infections, which may add bricks to current knowledge on HPV infection and lay the foundation for novel treatment/prevention development.
摘要：
阴道微生物组（VM）与人乳头瘤病毒（HPV）感染和进展有关，但要彻底了解HPV感染之间的关系，和VM需要阐明。2022年8月至12月，对接受常规妇科检查的妇女进行HPV感染筛查。收集HPV变异体的分布和临床特征。然后，共纳入185名参与者，分为HPV阴性（HC），高危型HPV（H），低危型HPV(L),多种高危型HPV（HH），高-低危型HPV（HL）混合组。从这185名参与者的阴道中部收集样品，并进行16SrDNA测序（V3-V4区域）。在712名HPV阳性女性中，前3位最常见的基因型是HPV52,HPV58和HPV16.在微生物学研究的185名参与者中，HC组的β多样性与HPV阳性组差异有统计学意义(P<0.001)。LEfSe分析显示，乳杆菌是H组的潜在生物标志物，L组则为crispatus。关于HPV阳性患者，宫颈病变患者的α多样性明显低于正常宫颈患者（P<0.05）。差异丰度分析显示，宫颈病变患者的詹氏乳杆菌明显减少（P<0.001）。进一步的群落状态类型（CST）聚类显示，CSTIV在HC组中比其他类型更常见（P<0.05）。H组CSTⅠ高于CSTⅣ(P<0.05)。不同的HPV感染具有不同的阴道微生物组特征。HPV感染可能导致乳杆菌属的失衡。引起宫颈病变.
目的：在本研究中，我们首先调查了中国南方不同HPV基因型的流行情况，这可以为HPV疫苗接种提供更多信息。然后，共185名受试者从HPV阴性，高风险,低风险,多重hr-hrHPV感染，和混合hr-lrHPV感染人群，探讨阴道微生物组的变化。这项研究表明，HPV52，HPV58和HPV16是中国南方最普遍的高危变种。此外,高危型HPV感染以乳杆菌为特征，而低危型HPV感染是由卷曲乳杆菌感染。进一步的亚组分析显示，在宫颈病变患者中，詹氏乳杆菌明显减少。最后,CST聚类显示CSTIV是HC组中最常见的类型，而CSTⅠ在H组中占最多。一句话,这项研究首次系统地分析了不同HPV感染的阴道微生物组，这可能会增加有关HPV感染的现有知识，并为新型治疗/预防的发展奠定基础。
目的：在本研究中，我们首先调查了中国南方不同HPV基因型的流行情况，这可以为HPV疫苗接种提供更多信息。然后，共185名受试者从HPV阴性，高风险,低风险,多重hr-hrHPV感染，和混合hr-lrHPV感染人群，探讨阴道微生物组的变化。这项研究表明，HPV52，HPV58和HPV16是中国南方最普遍的高危变种。此外,高危型HPV感染以乳杆菌为特征，而低危型HPV感染是由卷曲乳杆菌感染。进一步的亚组分析显示，在宫颈病变患者中，詹氏乳杆菌明显减少。最后,CST聚类显示CSTIV是HC组中最常见的类型，而CSTⅠ在H组中占最多。一句话,这项研究首次系统地分析了不同HPV感染的阴道微生物组，这可能会增加有关HPV感染的现有知识，并为新型治疗/预防的发展奠定基础。
