Abstract：
In response to the heightened risk of bacterial diseases in fish farms caused by increased demand for fish consumption and subsequent overcrowding, researchers are currently investigating the efficacy and residue management of oxolinic acid (OA) as a treatment for bacterial infections in fish. This research is crucial for gaining a comprehensive understanding of the pharmacokinetics of OA. The present study investigates pharmacokinetics of OA in juvenile rainbow trout. The fish were given a 12 mg kg-1 dose of OA through their feed, and tissue samples were collected of the liver, kidney, gill, intestine, muscle, and plasma for analysis using LC-MS/MS. The highest concentrations of the drug were found in the gill (4096.55 μg kg-1) and intestine (11592.98 μg kg-1), with significant absorption also seen in the liver (0.36 L/h) and gill (0.07 L/h) (p < 0.05). The liver (0.21 L/h) and kidney (0.03 L/h) were found to be the most efficient (p < 0.05) at eliminating the drug. The study also confirmed the drug antimicrobial effectiveness against several bacterial pathogens, including Shewanella xiamenensis (0.25 μg mL-1), Lactococcus garvieae (1 μg mL-1), and Chryseobacterium aquaticum (4 μg mL-1). The study concludes significant variations among different fish tissues, with higher concentrations and longer half-lives observed in the kidney and intestine. The lowest MIC value recorded against major bacterial pathogens demonstrated its therapeutic potential in aquaculture. It also emphasizes the importance of understanding OA pharmacokinetics to optimize antimicrobial therapy in aquaculture.
摘要：
为应对因鱼类消费需求增加和随后的过度拥挤而导致的养鱼场细菌性疾病风险增加，目前,研究人员正在研究二甲酚酸(OA)治疗鱼类细菌感染的疗效和残留管理.这项研究对于全面了解OA的药代动力学至关重要。本研究调查了OA在幼年虹鳟鱼中的药代动力学。通过饲料给鱼服用12mgkg-1剂量的OA，收集肝脏的组织样本，肾,吉尔,肠,肌肉，和使用LC-MS/MS分析的血浆在g（4096.55μgkg-1）和肠道（11592.98μgkg-1）中发现了最高浓度的药物，在肝脏（0.36L/h）和g（0.07L/h）中也观察到显着的吸收（p<0.05）。发现肝脏（0.21L/h）和肾脏（0.03L/h）在消除药物方面最有效（p<0.05）。该研究还证实了该药物对几种细菌病原体的抗菌效果，包括厦门希瓦氏菌（0.25μgmL-1），链球菌（1μgmL-1），和水金黄杆菌（4μgmL-1）。该研究得出了不同鱼类组织之间的显着差异，在肾脏和肠道中观察到更高的浓度和更长的半衰期。针对主要细菌病原体的最低MIC值表明了其在水产养殖中的治疗潜力。它还强调了了解OA药代动力学以优化水产养殖中抗微生物治疗的重要性。
