Abstract：
Single-cell RNA sequencing (scRNA-seq) has transformed our understanding of immune-mediated arthritis, which comprises rheumatoid arthritis and spondyloarthritis. This review outlines the key findings and advancements in scRNA-seq studies focused on the pathogenesis of autoimmune arthritis and its clinical application. In rheumatoid arthritis, scRNA-seq has elucidated the heterogeneity among synovial fibroblasts and immune cell subsets in inflammatory sites, offering insights into disease mechanisms and the differences in treatment responses. Various studies have identified distinct synovial fibroblast subpopulations, such as THY1+ inflammatory and THY1- destructive fibroblasts. Furthermore, scRNA-seq has revealed diverse T cell profiles in the synovium, including peripheral helper T cells and clonally expanded CD8+ T cells, shedding light on potential therapeutic targets and predictive markers of treatment response. Similarly, in spondyloarthritis, particularly psoriatic arthritis and ankylosing spondylitis, scRNA-seq studies have identified distinct cellular profiles associated with disease pathology. Challenges such as cost and sample size limitations persist, but collaborative efforts and utilization of public databases hold promise for overcoming these obstacles. Overall, scRNA-seq emerges as a powerful tool for dissecting cellular heterogeneity and driving precision medicine in immune-mediated arthritis.
摘要：
单细胞RNA测序（scRNA-seq）改变了我们对免疫介导的关节炎的理解，包括类风湿性关节炎和脊柱关节炎。本文概述了scRNA-seq研究的主要发现和进展，重点关注自身免疫性关节炎的发病机制及其临床应用。在类风湿性关节炎中，scRNA-seq已经阐明了炎症部位滑膜成纤维细胞和免疫细胞亚群之间的异质性，提供对疾病机制和治疗反应差异的见解。各种研究已经确定了不同的滑膜成纤维细胞亚群，如THY1+炎性和THY1-破坏性成纤维细胞。此外，scRNA-seq揭示了滑膜中不同的T细胞谱，包括外周辅助性T细胞和克隆扩增的CD8+T细胞，揭示潜在的治疗靶点和治疗反应的预测标志物。同样，在脊柱关节炎中，特别是银屑病关节炎和强直性脊柱炎,scRNA-seq研究已经确定了与疾病病理学相关的不同细胞谱。成本和样本量限制等挑战依然存在，但是合作努力和利用公共数据库有望克服这些障碍。总的来说,scRNA-seq成为一种强大的工具，用于在免疫介导的关节炎中解剖细胞异质性和驱动精准医学。
