UNASSIGNED: Samples were collected from the Hospital patients with stable angina pectoris (SAP). Peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll density gradient and analyzed via flow cytometry. IL-10, IL-6, and IL-17 levels in cell culture supernatant were measured using ELISA. Data were expressed as mean ± SEM and analyzed with statistical software.
UNASSIGNED: Results indicate that only patients exhibited reduced Treg and IL-10 levels after high-dose ox-LDL treatment. Significant IL-6 reduction was observed in both NCA and SA groups after high-dose n-LDL and low/high ox-LDL treatments compared to untreated PBMCs.
UNASSIGNED: Future research will explore n-LDL and ox-LDL effects on Th17/Treg immune responses within a specific cytokine environment known for inducing inflammation, assessing their potential role in atherosclerosis progression.
■从患有稳定型心绞痛(SAP)的医院患者收集样品。使用Ficoll密度梯度分离外周血单核细胞(PBMC)并通过流式细胞术分析。使用ELISA测量细胞培养上清液中的IL-10、IL-6和IL-17水平。数据表示为平均值±SEM,并用统计软件进行分析。
■结果表明,在高剂量ox-LDL治疗后,只有患者表现出Treg和IL-10水平降低。与未处理的PBMC相比,在高剂量n-LDL和低/高ox-LDL处理后,在NCA和SA组中均观察到显著的IL-6降低。
■未来的研究将探讨n-LDL和ox-LDL对Th17/Treg免疫反应的影响,在已知诱导炎症的特定细胞因子环境中,评估它们在动脉粥样硬化进展中的潜在作用。