Abstract：
Management of diarrhoea in horses is usually non-specific and supportive. Faecal microbiota transplantations (FMT) are used to manage dysbiosis in horses with diarrhoea. There are few studies investigating the effects of storage on prepared FMT solutions. This study was an in vitro non-randomised controlled experiment that investigated the effects of FMT solution preparation and storage on the faecal microbiota. Fresh faeces were collected from five healthy adult horses and used for DNA extraction and preparation of FMT. From each FMT, seven aliquots were collected and DNA was extracted immediately after FMT preparation (0 hr), after storage at 4 °C for 24, 48 or 72 hours, and after storage at -20°C for 7 days, 14 days or 28 days. The extracted DNA was used for 16 S rRNA gene sequencing. The relative abundance, alpha diversity and beta diversity between fresh faeces and FMT 0 hr showed no differences (P ≥ 0.05). There were minimal changes in the microbiota of FMT stored at 4°C for up to 72 hours and -20°C for up to 28 days. The results of this study indicate that preparation of equine FMT solution has minimal effect on the microbiota in comparison to fresh faeces. FMT solution can be stored at 4°C for up to 3 days and -20°C for 28 days without major change in microbiota.
摘要：
马腹泻的管理通常是非特异性和支持性的。粪便微生物群移植（FMT）用于管理腹泻马的生态失调。很少有研究研究储存对制备的FMT溶液的影响。这项研究是一项体外非随机对照实验，研究了FMT溶液制备和储存对粪便微生物群的影响。从五匹健康成年马中收集新鲜粪便，并用于DNA提取和FMT制备。从每个FMT，收集七个等分试样，并在FMT制备后立即提取DNA（0小时），在4℃下储存24、48或72小时后，在-20°C下储存7天后，14天或28天。提取的DNA用于16SrRNA基因测序。相对丰度，新鲜粪便和FMT0hr之间的α多样性和β多样性无差异（P≥0.05）。在4°C储存长达72小时和在-20°C储存长达28天的FMT的微生物群变化最小。这项研究的结果表明，与新鲜粪便相比，马FMT溶液的制备对微生物群的影响最小。FMT溶液可以在4°C下储存长达3天，在-20°C下储存28天，而不会发生微生物群的重大变化。
