Abstract：
Light is a vital environmental factor that promotes the growth and development of edible fungi mycelium. Under white light, the mycelium color of Sanghuangporus vaninii shifts during its growth stages. To investigate the impact of visible light on mycelial morphogenesis, a comparative transcriptomic analysis was conducted. This analysis revealed the molecular processes that underpin mycelial growth and development in S. vaninii when cultured in both darkness and light conditions. From the analysis, 13,643 genes were aligned using Illumina raw reads. Of these, 596 genes exhibited significant expression changes under white light exposure. Specifically, 226 genes were upregulated and 370 downregulated, spanning 55 different metabolic pathways. We further classified differentially expressed genes (DEGs), these genes play roles in photomorphogenesis, signal transduction, carbohydrate metabolism, and melanin production, among other processes. Some are also implicated in cell cycle regulation and the differential expression of respiratory functions. The validation of the differentially expressed transcripts using qRT-PCR showed complete agreement with RNA-Seq data for 9 transcripts. Meanwhile, the light had an inhibitory effect on the bioactive components in S. vaninii. These findings offer valuable insights into the transcriptional shifts and molecular mechanisms driving the color change in S. vaninii under light exposure, providing a basis for further research into mechanisms of light-response regulation.
摘要：
光照是促进食用菌菌丝体生长发育的重要环境因素。在白光下,香菇的菌丝体颜色在其生长阶段发生变化。探讨可见光对菌丝形态发生的影响,进行了比较转录组学分析.该分析揭示了在黑暗和光照条件下培养时支持S.vaninii菌丝生长和发育的分子过程。从分析来看,使用Illumina原始读数比对13,643个基因。其中,596个基因在白光照射下表现出显著的表达变化。具体来说,226个基因上调，370个基因下调，跨越55种不同的代谢途径。我们进一步分类了差异表达基因(DEGs)，这些基因在光形态发生中起作用，信号转导，碳水化合物代谢，和黑色素的产生，在其他过程中。一些还涉及细胞周期调节和呼吸功能的差异表达。使用RT-qPCR对差异表达的转录物的验证显示与9个转录物的RNA-Seq数据完全一致。同时,光照对Vaninii中的生物活性成分有抑制作用。这些发现提供了有价值的见解转录变化和分子机制驱动的颜色变化在光照下的瓦尼尼。光反应调控机制的进一步研究提供了依据。
