Abstract：
The pistil is the most important organ for fertilization in flowering plants, and the stigmatic papilla cells are responsible for pollen acceptance and pollen tube germination. Arabidopsis plants possess dry stigmas exhibiting high selectivity for compatible pollen. When compatible pollens are recognized and accepted by stigmatic papilla cells, water and nutrients are then transported from the stigma to pollen grains through the secretory pathway. Here, we present light microscopy-based methods for investigating autophagy and senescence of stigmatic papilla cells. These methods include the assessment of viability of stigmatic papilla cells using dual staining with fluorescein diacetate/propidium iodide, as well as the examination of vacuolar-accumulated proteins during stigma senescence. These methods can be used to understand the functions of the stigma tissue from a subcellular perspective.
摘要：
雌蕊是开花植物中最重要的受精器官，柱头乳头细胞负责花粉接受和花粉管萌发。拟南芥植物具有干燥的柱头，对相容的花粉表现出高选择性。当相容的花粉被柱头乳头细胞识别和接受时，然后，水和营养物质通过分泌途径从柱头运输到花粉粒。这里,我们提出了基于光学显微镜的方法来研究柱头乳头细胞的自噬和衰老。这些方法包括使用荧光素二乙酸盐/碘化丙啶双重染色评估柱头乳头细胞的活力，以及柱头衰老过程中液泡积累的蛋白质的检查。这些方法可用于从亚细胞角度理解柱头组织的功能。
