Abstract：
Protein secretion and vacuole formation are vital processes in plant cells, playing crucial roles in various aspects of plant development, growth, and stress responses. Multiple regulators have been uncovered to be involved in these processes. In animal cells, the transcription factor TFEB has been extensively studied and its role in lysosomal biogenesis is well understood. However, the transcription factors governing protein secretion and vacuole formation in plants remain largely unexplored. In recent years, an increasing number of bioinformatics databases and tools have been developed, facilitating computational prediction and analysis of the function of genes or proteins in specific cellular processes. Leveraging these resources, this chapter aims to provide practical guidance on how to effectively utilize these existing databases and tools for the analysis of key transcription factors involved in regulating protein secretion and vacuole formation in plants, with a particular focus on Arabidopsis and other higher plants. The findings from this analysis can serve as a valuable resource for future experimental investigations and the development of targeted strategies to manipulate protein secretion and vacuole formation in plants.
摘要：
蛋白质分泌和液泡形成是植物细胞的重要过程,在植物发育的各个方面发挥着至关重要的作用，增长,和应激反应。已经发现在这些过程中涉及多个调节器。在动物细胞中,转录因子TFEB已被广泛研究，其在溶酶体生物发生中的作用已被充分理解。然而,植物中控制蛋白质分泌和液泡形成的转录因子仍未被研究。近年来,已经开发了越来越多的生物信息学数据库和工具，促进特定细胞过程中基因或蛋白质功能的计算预测和分析。利用这些资源，本章旨在为如何有效利用这些现有的数据库和工具分析涉及植物蛋白质分泌和液泡形成调节的关键转录因子提供实用指导。特别关注拟南芥和其他高等植物。该分析的发现可作为未来实验研究和开发靶向策略以操纵植物中的蛋白质分泌和液泡形成的宝贵资源。
