Abstract：
The selective degradation of nuclear components via autophagy, termed nucleophagy, is an essential process observed from yeasts to mammals and crucial for maintaining nucleus homeostasis and regulating nucleus functions. In the budding yeast Saccharomyces cerevisiae, nucleophagy occurs in two different manners: one involves autophagosome formation for the sequestration and vacuolar transport of nucleus-derived vesicles (NDVs), and the other proceeds with the invagination of the vacuolar membrane for the uptake of NDVs into the vacuole, termed macronucleophagy and micronucleophagy, respectively. This chapter describes methods to analyze and quantify activities of these nucleophagy pathways in yeast.
摘要：
核成分通过自噬的选择性降解，称为核吞噬，是从酵母到哺乳动物观察到的重要过程，对于维持核稳态和调节核功能至关重要。在酿酒酵母中，核吞噬以两种不同的方式发生：一种涉及自噬体的形成，用于核衍生囊泡（NDV）的隔离和液泡运输，另一个是液泡膜内陷，将NDV吸收到液泡中，称为大核自噬和微核自噬，分别。本章介绍了分析和量化酵母中这些核吞噬途径活性的方法。
