Abstract：
This study evaluated the use of the GnRH agonist hormone, deslorelin, to control the follicular population before initiating multiple ovulation and embryo transfer (MOET) treatment. Twenty-four cross-bred Santa Inês ewes, aged between 2 and 4 years, were randomly assigned to either a control group (n = 11) or a treated group (n = 13). All ewes received an intravaginal device containing 60 mg of medroxyprogesterone acetate on day 0, and a new device on day 7, which remained in place until day 14. Additionally, the ewes were administered 125 μg of cloprostenol on day 7. The superovulatory treatment involved administering 200 mg of pFSH, divided into eight decreasing doses at 12-h intervals starting on day 12. On day 14, 300 IU of eCG was administered. In the deslorelin group, three doses of 100 μg of deslorelin were administered starting on day 3 after the insertion of the vaginal device, with subsequent doses given at 72-h and 144-h intervals. Natural mating was performed 36 h after the removal of the progesterone implant using males with proven fertility. Embryo collection took place on the 6th day after mating, and the recovered structures were quantified and evaluated for quality and developmental stage. Transrectal ultrasonography was conducted on days 12, 16 and 21 to evaluate the ovaries, specifically to assess the ovarian follicular population and the presence of the corpus luteum. Ewes in the control group had higher embryo recovery rates (p < .01) compared to the treated group (5.2 ± 0.8 vs. 1.1 ± 0.8), with differences observed primarily in the number of morulae. The number of corpus luteum observed during the laparotomy on day 21 was significantly higher (p < .01) in the control group (10.44 vs. 4.5 corpus luteum per ewe). Yet, the treated group had a significantly higher number of follicles (p < .05) on the first day of pFSH application (5.5 vs. 3.0 follicles per ewe). In conclusion, although the inclusion of deslorelin in the superovulation protocol resulted in increased synchronization of oestrus and follicle number, it did not lead to an increase in the number of corpus luteum or harvested embryos.
摘要：
这项研究评估了GnRH激动剂激素的使用，deslorelin,在启动多次排卵和胚胎移植（MOET）治疗之前控制卵泡群。二十四只杂交的圣塔因斯母羊，年龄在2至4岁之间，被随机分配到对照组（n=11）或治疗组（n=13）。所有母羊在第0天接受含有60mg醋酸甲羟孕酮的阴道内装置,并在第7天接受新装置,其保持原位直至第14天。此外,在第7天，母羊服用125μg的氯前列醇。超排卵治疗包括给予200毫克pFSH，从第12天开始，以12小时的间隔分为8次递减剂量。在第14天,施用300IU的eCG。在deslorelin组,从插入阴道装置后第3天开始，给予三剂100μg地洛瑞林，随后的剂量以72小时和144小时的间隔给予。在去除孕酮植入物后36小时，使用具有生育能力的雄性进行自然交配。胚胎采集发生在交配后的第6天,并对恢复的结构进行了量化和质量和发育阶段评估。在第12、16和21天进行经直肠超声检查以评估卵巢，特别是评估卵巢卵泡群和黄体的存在。与治疗组相比，对照组的母羊具有更高的胚胎恢复率（p<.01）（5.2±0.8vs.1.1±0.8),主要观察到桑树数量的差异。在第21天的剖腹手术中观察到的黄体数量在对照组中明显更高（p<0.01）（10.44vs.4.5黄体/母羊)。然而，在应用pFSH的第一天，治疗组的卵泡数量明显更高（p<.05）（5.5vs.每母羊3.0个卵泡）。总之,尽管在超排卵方案中包含地洛瑞林导致发情和卵泡数量同步增加，它没有导致黄体或收获的胚胎数量增加。
