PDF(Pubmed)
Abstract：
BACKGROUND: Residual ridge resorption presents obstacles in prosthodontic treatment, affecting denture stability and the success of dental implants. Genetic elements, specifically the single nucleotide polymorphism (SNP) 1772C>T variant within the hypoxia-inducible factor 1 subunit alpha (HIF-1α) gene, are hypothesized to contribute to residual ridge resorption progression. Nevertheless, its impact remains insufficiently investigated, especially within the context of South Indian populations. We sought to investigate the connection between SNP 1772C>T and residual ridge resorption (RRR) among fully edentulous individuals, considering demographic factors, genotyping methodologies, and statistical evaluations.
METHODS: In a prospective case-control study, we recruited 100 completely edentulous participants from South India. Participants were categorized based on alveolar ridge height. Saliva samples were non-invasively collected for DNA extraction, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was employed to determine genotype distribution using the HphI restriction enzyme. The statistical evaluations comprised the utilization of chi-square and Fisher\'s exact tests.
RESULTS: We observed no significant variations in genotype distributions between the case and control cohorts (CT: p=0.24; CC: p=0.65; TT: p=0.30). The heterozygous genotype CT was prevalent in both groups.
CONCLUSIONS: Although we did not observe significant associations between SNP 1772C>T and RRR, our findings imply a genetic predisposition to residual ridge resorption that warrants further exploration. Variations in genetic susceptibility across ethnicities and the influence of other genetic variants on residual ridge resorption require additional investigation. This study lays the groundwork for personalized prosthodontic care by highlighting the potential of genetic analysis in routine dental practice to improve treatment strategies.
METHODS: In a prospective case-control study, we recruited 100 completely edentulous participants from South India. Participants were categorized based on alveolar ridge height. Saliva samples were non-invasively collected for DNA extraction, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was employed to determine genotype distribution using the HphI restriction enzyme. The statistical evaluations comprised the utilization of chi-square and Fisher\'s exact tests.
RESULTS: We observed no significant variations in genotype distributions between the case and control cohorts (CT: p=0.24; CC: p=0.65; TT: p=0.30). The heterozygous genotype CT was prevalent in both groups.
CONCLUSIONS: Although we did not observe significant associations between SNP 1772C>T and RRR, our findings imply a genetic predisposition to residual ridge resorption that warrants further exploration. Variations in genetic susceptibility across ethnicities and the influence of other genetic variants on residual ridge resorption require additional investigation. This study lays the groundwork for personalized prosthodontic care by highlighting the potential of genetic analysis in routine dental practice to improve treatment strategies.
摘要：
背景：残余牙脊吸收在修复治疗中存在障碍，影响义齿稳定性和牙种植体的成功。遗传因子,特别是缺氧诱导因子1亚基α（HIF-1α）基因内的单核苷酸多态性（SNP）1772C>T变异，被假设为有助于残余脊吸收进展。然而，其影响仍未得到充分调查，特别是在南印度人口的背景下。我们试图调查SNP1772C>T与完全无牙个体中残余脊吸收（RRR）之间的联系，考虑到人口因素，基因分型方法，和统计评估。
方法：在一项前瞻性病例对照研究中，我们从印度南部招募了100名完全无牙的参与者.参与者根据牙槽脊高度进行分类。非侵入性收集唾液样本进行DNA提取，和聚合酶链反应限制性片段长度多态性（PCR-RFLP）分析用于确定使用HphI限制性内切酶的基因型分布。统计评价包括卡方检验和Fisher精确检验。
结果：我们观察到病例组和对照组之间基因型分布没有显著差异（CT：p=0.24；CC：p=0.65；TT：p=0.30）。杂合基因型CT在两组中普遍存在。
结论：尽管我们没有观察到SNP1772C>T和RRR之间的显著关联，我们的发现暗示了残余脊吸收的遗传易感性，值得进一步探索。不同种族的遗传易感性变化以及其他遗传变异对残留脊吸收的影响需要进一步研究。这项研究通过强调遗传分析在常规牙科实践中改善治疗策略的潜力，为个性化修复护理奠定了基础。
方法：在一项前瞻性病例对照研究中，我们从印度南部招募了100名完全无牙的参与者.参与者根据牙槽脊高度进行分类。非侵入性收集唾液样本进行DNA提取，和聚合酶链反应限制性片段长度多态性（PCR-RFLP）分析用于确定使用HphI限制性内切酶的基因型分布。统计评价包括卡方检验和Fisher精确检验。
结果：我们观察到病例组和对照组之间基因型分布没有显著差异（CT：p=0.24；CC：p=0.65；TT：p=0.30）。杂合基因型CT在两组中普遍存在。
结论：尽管我们没有观察到SNP1772C>T和RRR之间的显著关联，我们的发现暗示了残余脊吸收的遗传易感性，值得进一步探索。不同种族的遗传易感性变化以及其他遗传变异对残留脊吸收的影响需要进一步研究。这项研究通过强调遗传分析在常规牙科实践中改善治疗策略的潜力，为个性化修复护理奠定了基础。
