PDF(Pubmed)
Abstract：
Necrotic enteritis (NE) is a severe gastrointestinal disease that poses a significant threat to the poultry industry. It leads to progressive damage to the small intestine, reduced performance, increased mortality rates, and substantial economic losses. With the removal of antimicrobial agents from chicken feed, there is an urgent need to find alternative approaches for NE control. Various approaches, including vaccination, prebiotics, probiotics, and plant-derived products, have been utilized to address NE in poultry management. To evaluate the efficacy of these preventive measures against NE, successful induction of NE is crucial to observe effects of these approaches in related studies. This study presents a comprehensive overview of the methods and approaches utilized for NE reproduction in related studies from 2004 to 2023. These considerations are the careful selection of a virulent Clostridium perfringens strain, preparation of challenge inoculum, choice of time and the route for challenge inoculum administration, and utilization of one or more predisposing factors to increase the rate of NE occurrence in birds under experiment. We also reviewed the different systems used for lesion scoring of NE-challenged birds. By gaining clarity on these fundamental parameters, researchers can make informed decisions regarding the selection of the most appropriate NE experimental design in their respective studies.
摘要：
坏死性肠炎（NE）是一种严重的胃肠道疾病，对家禽业构成重大威胁。它会导致小肠的进行性损伤，降低性能，死亡率上升，和巨大的经济损失。随着鸡饲料中抗菌剂的去除，迫切需要找到控制NE的替代方法。各种方法，包括疫苗接种，益生元,益生菌，和植物衍生产品，已被用来解决家禽管理中的NE问题。为了评估这些针对NE的预防措施的有效性，在相关研究中，成功诱导NE对于观察这些方法的效果至关重要。本研究全面概述了2004年至2023年相关研究中用于NE繁殖的方法和方法。这些考虑因素是仔细选择一种毒力的产气荚膜梭菌菌株，制备挑战接种物，选择时间和途径的挑战接种，并利用一种或多种诱发因素来增加实验中鸟类的NE发生率。我们还回顾了用于NE攻击鸟类病变评分的不同系统。通过了解这些基本参数，研究人员可以在各自的研究中就选择最合适的NE实验设计做出明智的决定。
