Abstract：
During the pandemic, Favipiravir (FVP) and Molnupiravir (MPV) have been widely used for COVID-19 treatment, leading to their presence in the environment. A green synchronous spectrofluorimetric method was developed to simultaneously detect them in environmental water, human plasma, and binary mixtures. Maximum fluorescence intensity was achieved at pH 8, with MPV exhibiting two peaks at 300 and 430 nm, and FVP showing one peak at 430 nm. A fluorescence subtraction method effectively removed interference, enabling direct determination of MPV at 300 nm and FVP at 430 nm. The method showed linearity within 2-13 ng/mL for FVP and 50-600 ng/mL for MPV, with recoveries of 100.35% and 100.12%, respectively. Limits of detection and quantification were 0.19 and 0.57 ng/mL for FVP and 10.52 and 31.88 ng/mL for MPV. Validation according to ICH and FDA guidelines yielded acceptable results. The method demonstrated good recoveries of FVP and MPV in pharmaceuticals, tap water and Nile water (99.62% ± 0.96% and 99.69% ± 0.64%) as per ICH guidelines and spiked human plasma (94.87% ± 2.111% and 94.79% ± 1.605%) following FDA guidelines, respectively. Its environmental friendliness was assessed using Green Analytical Procedure Index (GAPI) and the Analytical Greenness Metric (AGREE) tools.
摘要：
大流行期间,Favipiravir（FVP）和Molnupiravir（MPV）已广泛用于COVID-19治疗，导致他们在环境中的存在。开发了一种绿色同步荧光光谱法，用于在环境水中同时检测它们，人血浆,和二元混合物。最大荧光强度在pH8时达到，MPV在300和430nm处显示两个峰，和FVP在430nm处显示一个峰。一种有效去除干扰的荧光减法,能够直接测定300nm的MPV和430nm的FVP。该方法显示FVP在2-13ng/mL范围内的线性，MPV在50-600ng/mL范围内，回收率分别为100.35%和100.12%,分别。FVP的检测和定量极限为0.19和0.57ng/mL，MPV为10.52和31.88ng/mL。根据ICH和FDA指南进行的验证产生了可接受的结果。该方法证明了药物中FVP和MPV的良好回收率，根据ICH指南，自来水和尼罗河水（99.62％±0.96％和99.69％±0.64％）以及符合FDA指南的人血浆（94.87％±2.111%和94.79％±1.605%），分别。使用绿色分析程序指数（GAPI）和分析绿色度量（AGREE）工具评估了其环境友好性。
