Abstract：
The healing of diabetic wounds has long been a significant challenge in the field of medicine. The elevated sugar levels surrounding diabetic wounds create a conducive environment for harmful bacterial growth, resulting in purulent infections that impede the healing process. Thus, the development of a biomaterial that can enhance the healing of diabetic wounds holds great importance. This study developed electrospun dressings for wound healing by combining traditional Chinese medicine and clay. The study utilized electrospinning technology to prepare polyvinyl alcohol (PVA) nanofiber membranes containing ASB and HNTs. These ASB@HNTs-PVA nanofiber membranes demonstrated rapid hemostasis, along with antibacterial and anti-inflammatory properties, facilitating the recovery of type 2 diabetic (T2D) wounds. Various analyses were conducted to assess the performance of the composite nanofiber membrane, including investigations into its biocompatibility and hemostatic abilities through antibacterial experiments, cell experiments, and mouse liver tail bleeding experiments. Western blot analysis confirmed that the composite nanofiber membrane could decrease the levels of inflammatory factors IL-1β and TNF-α. A type 2 diabetic mouse model was utilized, with wounds artificially induced on the backs of mice. Application of the nanofiber membrane to the wounds further confirmed its anti-inflammatory effects and ability to enhance wound healing in vivo.
摘要：
糖尿病伤口的愈合长期以来一直是医学领域的重大挑战。糖尿病伤口周围的糖水平升高为有害细菌生长创造了有利的环境，导致化脓性感染，阻碍愈合过程。因此，开发可增强糖尿病伤口愈合的生物材料非常重要。本研究通过结合中药和粘土开发了用于伤口愈合的电纺敷料。该研究利用静电纺丝技术制备了含ASB和HNTs的聚乙烯醇（PVA）纳米纤维膜。这些ASB@HNTs-PVA纳米纤维膜表现出快速止血，以及抗菌和抗炎特性，促进2型糖尿病（T2D）伤口的恢复。进行了各种分析以评估复合纳米纤维膜的性能，包括通过抗菌实验研究其生物相容性和止血能力，细胞实验,和小鼠肝尾出血实验。Westernblot分析证实复合纳米纤维膜可降低炎症因子IL-1β和TNF-α的水平。利用2型糖尿病小鼠模型，老鼠背上人工诱导的伤口。将纳米纤维膜应用于伤口进一步证实了其抗炎作用和增强体内伤口愈合的能力。
