Abstract：
Key wound environment parameters include pH, hydration, and the balance between tissue remodeling and deposition of new tissue. When prolonged inflammation is present, the proliferation phase of wound healing can be delayed because excessive protease production due to persistent inflammation can destroy newly formed tissue and prevent wounds from filling and reepithelializing.
To conduct an in vitro study of the ability of polygalacturonic acid (PG), a natural pectin derivative present in ripening fruit, to inhibit 3 destructive wound proteases and prevent dehydration in environments in which significant evaporation can occur.
In vitro enzyme inhibition assay kits were used to detect the ability of PG to inhibit key wound proteases matrix metalloproteinase (MMP)-2, MMP-9, and neutrophil elastase (NE). Transepidermal evaporative water loss from a polyvinyl alcohol skin substitute hydrogel was gravimetrically measured.
PG could partially inhibit MMP-2 (>50% inhibition relative to negative controls), MMP-9 (>50% inhibition relative to negative controls), and NE (>25% inhibition relative to negative controls) and thereby potentially blunt some of the destructive effects of excess proteases where prolonged inflammation is present. In an in vitro transepidermal evaporative water loss assay, PG also helped retain moisture and inhibited dehydration (>25% reduction relative to negative controls).
These findings suggest that PG can be a useful addition to ointments and dressings in wound care and warrants further in vivo testing.
To conduct an in vitro study of the ability of polygalacturonic acid (PG), a natural pectin derivative present in ripening fruit, to inhibit 3 destructive wound proteases and prevent dehydration in environments in which significant evaporation can occur.
In vitro enzyme inhibition assay kits were used to detect the ability of PG to inhibit key wound proteases matrix metalloproteinase (MMP)-2, MMP-9, and neutrophil elastase (NE). Transepidermal evaporative water loss from a polyvinyl alcohol skin substitute hydrogel was gravimetrically measured.
PG could partially inhibit MMP-2 (>50% inhibition relative to negative controls), MMP-9 (>50% inhibition relative to negative controls), and NE (>25% inhibition relative to negative controls) and thereby potentially blunt some of the destructive effects of excess proteases where prolonged inflammation is present. In an in vitro transepidermal evaporative water loss assay, PG also helped retain moisture and inhibited dehydration (>25% reduction relative to negative controls).
These findings suggest that PG can be a useful addition to ointments and dressings in wound care and warrants further in vivo testing.
摘要：
背景：关键的伤口环境参数包括pH，水合作用,以及组织重塑和新组织沉积之间的平衡。当存在长期炎症时，伤口愈合的增殖阶段可以延迟，因为由于持续的炎症导致的过度蛋白酶产生可以破坏新形成的组织并阻止伤口填充和再上皮化。
目的：进行聚半乳糖醛酸（PG）的体外研究，成熟果实中存在的天然果胶衍生物，抑制3种破坏性伤口蛋白酶并防止在可能发生显著蒸发的环境中脱水。
方法：采用体外酶抑制试剂盒检测PG对关键创伤蛋白酶基质金属蛋白酶(MMP)-2、MMP-9和中性粒细胞弹性蛋白酶(NE)的抑制能力。重量分析测量聚乙烯醇皮肤替代物水凝胶的经表皮蒸发失水。
结果：PG可以部分抑制MMP-2（相对于阴性对照，抑制>50％），MMP-9（相对于阴性对照，抑制>50%），和NE（相对于阴性对照，抑制>25％），从而潜在地减弱了存在长期炎症的过量蛋白酶的一些破坏性作用。在体外经表皮蒸发失水试验中，PG还有助于保持水分和抑制脱水(相对于阴性对照减少>25%)。
结论：这些研究结果表明，PG可以作为伤口护理中软膏和敷料的有用补充，并需要进一步的体内测试。
目的：进行聚半乳糖醛酸（PG）的体外研究，成熟果实中存在的天然果胶衍生物，抑制3种破坏性伤口蛋白酶并防止在可能发生显著蒸发的环境中脱水。
方法：采用体外酶抑制试剂盒检测PG对关键创伤蛋白酶基质金属蛋白酶(MMP)-2、MMP-9和中性粒细胞弹性蛋白酶(NE)的抑制能力。重量分析测量聚乙烯醇皮肤替代物水凝胶的经表皮蒸发失水。
结果：PG可以部分抑制MMP-2（相对于阴性对照，抑制>50％），MMP-9（相对于阴性对照，抑制>50%），和NE（相对于阴性对照，抑制>25％），从而潜在地减弱了存在长期炎症的过量蛋白酶的一些破坏性作用。在体外经表皮蒸发失水试验中，PG还有助于保持水分和抑制脱水(相对于阴性对照减少>25%)。
结论：这些研究结果表明，PG可以作为伤口护理中软膏和敷料的有用补充，并需要进一步的体内测试。
