PDF(Pubmed)
Abstract：
Insects and their gut bacteria form a tight and beneficial relationship, especially in utilization of host nutrients. The red turpentine beetle (RTB), a destructive and invasive pine pest, employs mutualistic microbes to facilitate its invasion success. However, the molecular mechanism underlying the utilization of nutrients remains unknown. In this study, we found that gut bacteria are crucial for the utilization of D-glucose, a main carbon source for RTB development. Downstream assays revealed that gut bacteria-induced gut hypoxia and the secretion of riboflavin are responsible for RTB development by regulating D-glucose transport via the activation of a hypoxia-induced transcription factor 1 (Hif-1α). Further functional investigations confirmed that Hif-1α mediates glucose transport by direct upregulation of two glucose transporters (ST10 and ST27), thereby promoting RTB development. Our findings reveal how gut bacteria regulate the development of RTB, and promote our understanding of the mutualistic relationship of animals and their gut bacteria.
摘要：
昆虫和它们的肠道细菌形成了紧密有益的关系，特别是在宿主营养的利用方面。红松节油甲虫（RTB），一种破坏性和侵入性的松树害虫，利用互惠的微生物来促进其入侵成功。然而,营养素利用的分子机制仍然未知。在这项研究中,我们发现肠道细菌对D-葡萄糖的利用至关重要，RTB开发的主要碳源。下游分析显示，肠道细菌诱导的肠道缺氧和核黄素的分泌是通过激活缺氧诱导的转录因子1（Hif-1α）调节D-葡萄糖转运而导致RTB发育的原因。进一步的功能研究证实，Hif-1α通过两个葡萄糖转运蛋白（ST10和ST27）的直接上调介导葡萄糖转运。从而促进RTB的发展。我们的发现揭示了肠道细菌如何调节RTB的发展，促进我们对动物和肠道细菌相互关系的理解。
