Abstract：
OBJECTIVE: To analyze the correlation between cerebrospinal fluid (CSF) interleukin-10 (IL-10) levels and the clinical characteristics in patients with vitreoretinal lymphoma (VRL).
METHODS: Retrospective observational case series.
METHODS: Forty-one patients were diagnosed as VRL and underwent lumbar puncture for CSF examination. Aqueous humor cytokine detection, vitreous cytopathologic analysis, monoclonal gene rearrangement, and flow cytometry were performed. The CSF was assessed through biochemical and cytologic examination, flow cytometry, and cytokine detection.
RESULTS: The median levels of aqueous humor IL-10 and IL-6 were 415.0 and 40.7 pg/mL. The median CSF levels of IL-10 and IL-6 were 35.7 and 3.5 pg/mL, respectively. IL-10 levels in CSF were higher than normal in 37 patients (90.2%) and higher in patients with intracranial lesions. The level of CSF IL-10 decreased after systemic treatment, and it rose before intracranial lesion onset or recurrence. The level of IL-10 in CSF was related to the duration of ocular symptoms, but was not related to the level of IL-10 in aqueous humor. There was no significant difference in CSF IL-10 levels between patients with and without anterior chamber inflammation or retinal lesions. In eyes with recurrent vitreoretinal lymphoma, the level of IL-10 in aqueous humor increased significantly, but there was no corresponding increase in the level of IL-10 in CSF.
CONCLUSIONS: CSF IL-10 is a potentially important biomarker in VRL, especially in the monitoring of intracranial lesions.
METHODS: Retrospective observational case series.
METHODS: Forty-one patients were diagnosed as VRL and underwent lumbar puncture for CSF examination. Aqueous humor cytokine detection, vitreous cytopathologic analysis, monoclonal gene rearrangement, and flow cytometry were performed. The CSF was assessed through biochemical and cytologic examination, flow cytometry, and cytokine detection.
RESULTS: The median levels of aqueous humor IL-10 and IL-6 were 415.0 and 40.7 pg/mL. The median CSF levels of IL-10 and IL-6 were 35.7 and 3.5 pg/mL, respectively. IL-10 levels in CSF were higher than normal in 37 patients (90.2%) and higher in patients with intracranial lesions. The level of CSF IL-10 decreased after systemic treatment, and it rose before intracranial lesion onset or recurrence. The level of IL-10 in CSF was related to the duration of ocular symptoms, but was not related to the level of IL-10 in aqueous humor. There was no significant difference in CSF IL-10 levels between patients with and without anterior chamber inflammation or retinal lesions. In eyes with recurrent vitreoretinal lymphoma, the level of IL-10 in aqueous humor increased significantly, but there was no corresponding increase in the level of IL-10 in CSF.
CONCLUSIONS: CSF IL-10 is a potentially important biomarker in VRL, especially in the monitoring of intracranial lesions.
摘要：
目的：分析玻璃体视网膜淋巴瘤(VRL)患者脑脊液白细胞介素-10(IL-10)水平与临床特征的相关性。
方法：回顾性观察病例系列。
方法：41例患者诊断为VRL，并进行腰椎穿刺脑脊液检查。房水细胞因子检测，玻璃体细胞病理学分析,单克隆基因重排，并进行流式细胞术。通过生化和细胞学检查评估脑脊液，流式细胞术,和细胞因子检测。
结果：房水IL-10和IL-6的中位数水平分别为415.0和40.7pg/mL。IL-10和IL-6的CSF水平中位数分别为35.7和3.5pg/mL，分别。37例患者脑脊液中IL-10水平高于正常值（90.2%），颅内病变患者高于正常值。全身治疗后脑脊液IL-10水平下降，在颅内病变发作或复发之前升高。脑脊液中IL-10水平与眼部症状持续时间有关,但与房水中IL-10水平无关。有和没有前房炎症或视网膜病变的患者之间的CSFIL-10水平没有显着差异。在复发性玻璃体视网膜淋巴瘤的眼中，房水IL-10水平显著升高,但CSF中IL-10水平没有相应增加。
结论：CSFIL-10是VRL中潜在的重要生物标志物，尤其是颅内病变的监测。
方法：回顾性观察病例系列。
方法：41例患者诊断为VRL，并进行腰椎穿刺脑脊液检查。房水细胞因子检测，玻璃体细胞病理学分析,单克隆基因重排，并进行流式细胞术。通过生化和细胞学检查评估脑脊液，流式细胞术,和细胞因子检测。
结果：房水IL-10和IL-6的中位数水平分别为415.0和40.7pg/mL。IL-10和IL-6的CSF水平中位数分别为35.7和3.5pg/mL，分别。37例患者脑脊液中IL-10水平高于正常值（90.2%），颅内病变患者高于正常值。全身治疗后脑脊液IL-10水平下降，在颅内病变发作或复发之前升高。脑脊液中IL-10水平与眼部症状持续时间有关,但与房水中IL-10水平无关。有和没有前房炎症或视网膜病变的患者之间的CSFIL-10水平没有显着差异。在复发性玻璃体视网膜淋巴瘤的眼中，房水IL-10水平显著升高,但CSF中IL-10水平没有相应增加。
结论：CSFIL-10是VRL中潜在的重要生物标志物，尤其是颅内病变的监测。
