Abstract：
Investigating the interactions between nanomaterials and the cells they are likely to encounter in vivo is a critical aspect of designing nanomedicines for imaging and therapeutic applications. Immune cells such as dendritic cells, macrophages, and myeloid derived suppressor cells have a frontline role in the identification and removal of foreign materials from the body, with interactions shown to be heavily dependent on variables such as nanoparticle size, charge, and surface chemistry. Interactions such as cellular association or uptake of nanoparticles can lead to diminished functionality or rapid clearance from the body, making it critical to consider these interactions when designing and synthesizing nanomaterials for biomedical applications ranging from drug delivery to imaging and biosensing. We investigated the interactions between PEGylated organosilica nanoparticles and naturally endocytic immune cells grown from stem cells in murine bone marrow. Specifically, we varied the particle size from 60 nm up to 1000 nm and investigated the effects of size on immune cell association, activation, and maturation with these critical gatekeeper cells. These results will help inform future design parameters for in vitro and in vivo biomedical applications utilizing organosilica nanoparticles.
摘要：
研究纳米材料与它们可能在体内遇到的细胞之间的相互作用是设计用于成像和治疗应用的纳米药物的关键方面。免疫细胞如树突状细胞,巨噬细胞，骨髓来源的抑制细胞在识别和清除体内异物方面具有前线作用，相互作用严重依赖于纳米颗粒尺寸等变量，charge,表面化学。相互作用，如细胞缔合或纳米颗粒的摄取可导致功能减弱或从体内快速清除，这使得在设计和合成从药物递送到成像和生物传感的生物医学应用的纳米材料时考虑这些相互作用变得至关重要。我们研究了聚乙二醇化的有机二氧化硅纳米颗粒与从小鼠骨髓中的干细胞生长的天然内吞免疫细胞之间的相互作用。具体来说,我们改变了颗粒大小从60纳米到1000纳米，并研究了大小对免疫细胞结合的影响，激活,和这些关键的看门人细胞的成熟。这些结果将有助于为利用有机二氧化硅纳米粒子的体外和体内生物医学应用提供未来的设计参数。
