Abstract：
Oxidative stress is a pivotal factor in the pathogenesis of various cardiovascular diseases. Rhodiola, a traditional Chinese medicine, is recognized for its potent antioxidant properties. Salidroside, a phenylpropanoid glycoside derived from Rhodiola rosea, has shown remarkable antioxidant capabilities. This study aimed to elucidate the potential protective mechanisms of Rhodiola and salidroside against H2O2-induced cardiac apoptosis in H9c2 cardiomyoblast cells. H9c2 cells were exposed to H2O2 for 4 h, and subsequently treated with Rhodiola or salidroside for 24 h. Cell viability and apoptotic pathways were assessed. The involvement of insulin-like growth factor 1 receptor (IGF1R) and the activation of extracellular regulated protein kinases 1/2 (ERK1/2) were investigated. H2O2 (100 μM) exposure significantly induced cardiac apoptosis in H9c2 cells. However, treatment with Rhodiola (12.5, 25, and 50 μg/mL) and salidroside (0.1, 1, and 10 nM) effectively attenuated H2O2-induced cytotoxicity and apoptosis. This protective effect was associated with IGF1R-activated phosphorylation of ERK1/2, leading to the inhibition of Fas-dependent proteins, HIF-1α, Bax, and Bak expression in H9c2 cells. The images from hematoxylin and eosin staining and immunofluorescence assays also revealed the protective effects of Rhodiola and salidroside in H9c2 cells against oxidative damage. Our findings suggest that Rhodiola and salidroside possess antioxidative properties that mitigate H2O2-induced apoptosis in H9c2 cells. The protective mechanisms involve the activation of IGF1R and subsequent phosphorylation of ERK1/2. These results propose Rhodiola and salidroside as potential therapeutic agents for cardiomyocyte cytotoxicity and apoptosis induced by oxidative stress in heart diseases. Future studies may explore their clinical applications in cardiac health.
摘要：
氧化应激是各种心血管疾病发病的关键因素。红景天,中药，以其有效的抗氧化性能而闻名。Salidroside,一种来源于红景天的苯丙素糖苷，表现出显著的抗氧化能力。本研究旨在阐明红景天和红景天苷对H2O2诱导的H9c2心肌细胞凋亡的潜在保护机制。H9c2细胞暴露于H2O24小时，随后用红景天或红景天苷处理24小时。评估细胞活力和凋亡途径。研究了胰岛素样生长因子1受体（IGF1R）的参与和细胞外调节蛋白激酶1/2（ERK1/2）的激活。H2O2(100μM)暴露显著诱导H9c2细胞的心脏凋亡。然而,红景天（12.5、25和50μg/mL）和红景天苷（0.1、1和10nM）的处理可有效减弱H2O2诱导的细胞毒性和细胞凋亡。这种保护作用与IGF1R激活的ERK1/2磷酸化有关，导致Fas依赖性蛋白的抑制，HIF-1α,Bax,和Bak在H9c2细胞中的表达。苏木精和曙红染色和免疫荧光测定的图像还揭示了红景天和红景天苷在H9c2细胞中对氧化损伤的保护作用。我们的发现表明，红景天和红景天苷具有抗氧化特性，可以减轻H2O2诱导的H9c2细胞凋亡。保护机制涉及IGF1R的激活和随后的ERK1/2的磷酸化。这些结果表明红景天和红景天苷是心脏疾病中氧化应激诱导的心肌细胞细胞毒性和凋亡的潜在治疗剂。未来的研究可能会探索其在心脏健康中的临床应用。
