PDF(Pubmed)
Abstract：
UNASSIGNED: Morphine exposure during pregnancy has detrimental effects on both the mother and her offspring, both during and after childbirth. This study aimed to investigate the impact of prenatal morphine exposure on rat pups and dams, specifically focusing on changes in Neuregulin-1 (Nrg-1)/ErbB4 gene expression, inflammation, and brain-derived neurotrophic factor (BDNF) levels.
UNASSIGNED: Twenty female rats were randomized into two experimental groups:1-Morphine Group: Dams received morphine throughout pregnancy. 2-Control Group: Dams received no interventions.At the end of gestation, blood samples were collected from the dams. Subsequently, dams and their pups underwent tissue collection from the cortical area of the brain to evaluate the following parameters: Interleukin-6 (IL-6), Interleukin-10 (IL-10), total antioxidant capacity (TAC), Malondialdehyde (MDA), and Brain-derived neurotrophic factor (BDNF).Additionally, RNA was extracted from the pup\'s cortical brain tissue for the assessment of gene expression levels of Neuregulin-1 (NRG-1) and ErbB-4 using quantitative real-time polymerase chain reaction (qrt-PCR).
UNASSIGNED: The molecular investigation revealed a decrease in NRG-1 and ErbB-4 expressions in the brain cortex of offspring exposed to morphine during prenatal development. Additionally, the levels of IL-6 and IL-10 in both the serum and brain of both the mothers and their offspring in the morphine group were significantly higher compared to the control group. The morphine-exposed group also exhibited significantly lower levels of TAC and higher levels of MDA, indicating increased oxidative stress. Furthermore, the levels of BDNF in the morphine group were significantly lower compared to the control group.
UNASSIGNED: Prenatal morphine exposure in rats has detrimental effects on both the dams and their offspring. This study demonstrates that prenatal morphine exposure disrupts critical molecular pathways involved in neurodevelopment, inflammation, oxidative stress, and neurotrophic signaling. These findings suggest that prenatal morphine exposure can have long-lasting consequences for the offspring, potentially contributing to neurodevelopmental disorders and other health issues later in life.
UNASSIGNED: Twenty female rats were randomized into two experimental groups:1-Morphine Group: Dams received morphine throughout pregnancy. 2-Control Group: Dams received no interventions.At the end of gestation, blood samples were collected from the dams. Subsequently, dams and their pups underwent tissue collection from the cortical area of the brain to evaluate the following parameters: Interleukin-6 (IL-6), Interleukin-10 (IL-10), total antioxidant capacity (TAC), Malondialdehyde (MDA), and Brain-derived neurotrophic factor (BDNF).Additionally, RNA was extracted from the pup\'s cortical brain tissue for the assessment of gene expression levels of Neuregulin-1 (NRG-1) and ErbB-4 using quantitative real-time polymerase chain reaction (qrt-PCR).
UNASSIGNED: The molecular investigation revealed a decrease in NRG-1 and ErbB-4 expressions in the brain cortex of offspring exposed to morphine during prenatal development. Additionally, the levels of IL-6 and IL-10 in both the serum and brain of both the mothers and their offspring in the morphine group were significantly higher compared to the control group. The morphine-exposed group also exhibited significantly lower levels of TAC and higher levels of MDA, indicating increased oxidative stress. Furthermore, the levels of BDNF in the morphine group were significantly lower compared to the control group.
UNASSIGNED: Prenatal morphine exposure in rats has detrimental effects on both the dams and their offspring. This study demonstrates that prenatal morphine exposure disrupts critical molecular pathways involved in neurodevelopment, inflammation, oxidative stress, and neurotrophic signaling. These findings suggest that prenatal morphine exposure can have long-lasting consequences for the offspring, potentially contributing to neurodevelopmental disorders and other health issues later in life.
摘要：
■怀孕期间的吗啡暴露对母亲及其后代都有不利影响，分娩期间和分娩后。本研究旨在探讨产前吗啡暴露对幼鼠和母鼠的影响,特别关注Neuregulin-1（Nrg-1）/ErbB4基因表达的变化，炎症,和脑源性神经营养因子（BDNF）水平。
■将20只雌性大鼠随机分为两个实验组：1-吗啡组：Dams在整个怀孕期间接受吗啡。2-对照组：水坝未接受干预。在妊娠结束时，从水坝收集血液样本。随后，从大脑皮层区域收集水坝及其幼崽的组织，以评估以下参数：白细胞介素-6（IL-6），白细胞介素-10(IL-10),总抗氧化能力(TAC)，丙二醛(MDA),脑源性神经营养因子(BDNF)。此外,使用定量实时聚合酶链反应（qrt-PCR）从幼崽的皮质脑组织中提取RNA，以评估Neuregulin-1（NRG-1）和ErbB-4的基因表达水平。
■分子研究显示，在产前发育过程中，暴露于吗啡的后代大脑皮层中NRG-1和ErbB-4的表达减少。此外,与对照组相比，吗啡组母亲及其后代的血清和大脑中IL-6和IL-10的水平显着升高。吗啡暴露组的TAC水平也显著降低，MDA水平升高，表明氧化应激增加。此外，吗啡组的BDNF水平明显低于对照组。
■大鼠的产前吗啡暴露对母鼠及其后代都有有害影响。这项研究表明，产前吗啡暴露破坏了参与神经发育的关键分子途径，炎症,氧化应激，和神经营养信号。这些发现表明，产前吗啡暴露会对后代产生长期的影响，在以后的生活中可能导致神经发育障碍和其他健康问题。
■将20只雌性大鼠随机分为两个实验组：1-吗啡组：Dams在整个怀孕期间接受吗啡。2-对照组：水坝未接受干预。在妊娠结束时，从水坝收集血液样本。随后，从大脑皮层区域收集水坝及其幼崽的组织，以评估以下参数：白细胞介素-6（IL-6），白细胞介素-10(IL-10),总抗氧化能力(TAC)，丙二醛(MDA),脑源性神经营养因子(BDNF)。此外,使用定量实时聚合酶链反应（qrt-PCR）从幼崽的皮质脑组织中提取RNA，以评估Neuregulin-1（NRG-1）和ErbB-4的基因表达水平。
■分子研究显示，在产前发育过程中，暴露于吗啡的后代大脑皮层中NRG-1和ErbB-4的表达减少。此外,与对照组相比，吗啡组母亲及其后代的血清和大脑中IL-6和IL-10的水平显着升高。吗啡暴露组的TAC水平也显著降低，MDA水平升高，表明氧化应激增加。此外，吗啡组的BDNF水平明显低于对照组。
■大鼠的产前吗啡暴露对母鼠及其后代都有有害影响。这项研究表明，产前吗啡暴露破坏了参与神经发育的关键分子途径，炎症,氧化应激，和神经营养信号。这些发现表明，产前吗啡暴露会对后代产生长期的影响，在以后的生活中可能导致神经发育障碍和其他健康问题。
